Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle
The NS2 protein of hepatitis C virus (HCV) plays a critical role in virus morphogenesis and infectivity. The crystal structure of the C-terminus of the NS2 protein (NS2 Pro) from the H77 strain indicates that NS2 Pro forms a homodimer. In this study,...
| Main Authors: | , , , |
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| Format: | Journal Article |
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2012
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| Online Access: | http://hdl.handle.net/20.500.11937/35882 |
| _version_ | 1848754615897554944 |
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| author | Gorzin, A. Ramsland, Paul Tachedjian, G. Gowans, E. |
| author_facet | Gorzin, A. Ramsland, Paul Tachedjian, G. Gowans, E. |
| author_sort | Gorzin, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The NS2 protein of hepatitis C virus (HCV) plays a critical role in virus morphogenesis and infectivity. The crystal structure of the C-terminus of the NS2 protein (NS2 Pro) from the H77 strain indicates that NS2 Pro forms a homodimer. In this study, using computational modelling, we identified residues at the NS2 Pro dimer interface that have a role in dimerization and confirmed their capacity to influence dimerization by expression studies. Our modelling analysis identified 22 residues at the NS2 Pro dimer interface that may be important for dimer formation. Based on the free binding energy, we selected the top five ranked mutations (V162A, M170A, I175A, D186A and I201A) for further study. Western blot analysis revealed that M170A, I175A, I201A, D186A and V162A resulted in a 4.0-, 3.2-, 3.0-, 2.8- and 1.5-fold increase, respectively, in the monomer/dimer ratio compared to wild type, confirming a role in homodimer formation or stability. Japanese Fulminant Hepatitis type 1 mutants expressing M170A, I175A, D186A and I201A demonstrated increasing defects in both RNA replication and the production of infectious virus compared to wild type. This study identified residues at the NS2 Pro dimer interface that modulate NS2 Pro homodimerization and demonstrated that abrogation of NS2 Pro homodimerization results in defects in HCV replication and release of infectious virus. © 2011 Blackwell Publishing Ltd. |
| first_indexed | 2025-11-14T08:43:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-35882 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:43:14Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-358822017-09-13T15:22:40Z Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle Gorzin, A. Ramsland, Paul Tachedjian, G. Gowans, E. The NS2 protein of hepatitis C virus (HCV) plays a critical role in virus morphogenesis and infectivity. The crystal structure of the C-terminus of the NS2 protein (NS2 Pro) from the H77 strain indicates that NS2 Pro forms a homodimer. In this study, using computational modelling, we identified residues at the NS2 Pro dimer interface that have a role in dimerization and confirmed their capacity to influence dimerization by expression studies. Our modelling analysis identified 22 residues at the NS2 Pro dimer interface that may be important for dimer formation. Based on the free binding energy, we selected the top five ranked mutations (V162A, M170A, I175A, D186A and I201A) for further study. Western blot analysis revealed that M170A, I175A, I201A, D186A and V162A resulted in a 4.0-, 3.2-, 3.0-, 2.8- and 1.5-fold increase, respectively, in the monomer/dimer ratio compared to wild type, confirming a role in homodimer formation or stability. Japanese Fulminant Hepatitis type 1 mutants expressing M170A, I175A, D186A and I201A demonstrated increasing defects in both RNA replication and the production of infectious virus compared to wild type. This study identified residues at the NS2 Pro dimer interface that modulate NS2 Pro homodimerization and demonstrated that abrogation of NS2 Pro homodimerization results in defects in HCV replication and release of infectious virus. © 2011 Blackwell Publishing Ltd. 2012 Journal Article http://hdl.handle.net/20.500.11937/35882 10.1111/j.1365-2893.2011.01504.x restricted |
| spellingShingle | Gorzin, A. Ramsland, Paul Tachedjian, G. Gowans, E. Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle |
| title | Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle |
| title_full | Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle |
| title_fullStr | Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle |
| title_full_unstemmed | Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle |
| title_short | Identification of residues involved in NS2 homodimerization and elucidation of their impact on the HCV life cycle |
| title_sort | identification of residues involved in ns2 homodimerization and elucidation of their impact on the hcv life cycle |
| url | http://hdl.handle.net/20.500.11937/35882 |