A high-throughput screen to identify novel calcineurin inhibitors
Calcineurin is a eukaryotic protein phosphatase important for many signalling and developmental processes in cells. Inhibitors of this enzyme are used clinically and there is interest in identifying novel inhibitors for therapeutic applications. This report describes a high-throughput assay that can...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier Inc
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/33306 |
| _version_ | 1848753909099659264 |
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| author | Margassery, L. Kennedy, J. O'Gara, Fergal Dobson, A. Morrissey, J. |
| author_facet | Margassery, L. Kennedy, J. O'Gara, Fergal Dobson, A. Morrissey, J. |
| author_sort | Margassery, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Calcineurin is a eukaryotic protein phosphatase important for many signalling and developmental processes in cells. Inhibitors of this enzyme are used clinically and there is interest in identifying novel inhibitors for therapeutic applications. This report describes a high-throughput assay that can be used to screen natural or chemical libraries of compounds to identify new calcineurin inhibitors. The microtitre plate assay is based on a yeast reporter strain and was validated with known inhibitors and tested in a pilot screen of bacterial extracts. |
| first_indexed | 2025-11-14T08:32:00Z |
| format | Journal Article |
| id | curtin-20.500.11937-33306 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:32:00Z |
| publishDate | 2012 |
| publisher | Elsevier Inc |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-333062017-09-13T15:31:39Z A high-throughput screen to identify novel calcineurin inhibitors Margassery, L. Kennedy, J. O'Gara, Fergal Dobson, A. Morrissey, J. Calcineurin is a eukaryotic protein phosphatase important for many signalling and developmental processes in cells. Inhibitors of this enzyme are used clinically and there is interest in identifying novel inhibitors for therapeutic applications. This report describes a high-throughput assay that can be used to screen natural or chemical libraries of compounds to identify new calcineurin inhibitors. The microtitre plate assay is based on a yeast reporter strain and was validated with known inhibitors and tested in a pilot screen of bacterial extracts. 2012 Journal Article http://hdl.handle.net/20.500.11937/33306 10.1016/j.mimet.2011.10.012 Elsevier Inc restricted |
| spellingShingle | Margassery, L. Kennedy, J. O'Gara, Fergal Dobson, A. Morrissey, J. A high-throughput screen to identify novel calcineurin inhibitors |
| title | A high-throughput screen to identify novel calcineurin inhibitors |
| title_full | A high-throughput screen to identify novel calcineurin inhibitors |
| title_fullStr | A high-throughput screen to identify novel calcineurin inhibitors |
| title_full_unstemmed | A high-throughput screen to identify novel calcineurin inhibitors |
| title_short | A high-throughput screen to identify novel calcineurin inhibitors |
| title_sort | high-throughput screen to identify novel calcineurin inhibitors |
| url | http://hdl.handle.net/20.500.11937/33306 |