Quantitative Real-Time PCR in aDNA Research

QR Code

Quantitative Real-Time PCR in aDNA Research

Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outlin...

Full description

Bibliographic Details
Main Authors:	Bunce, Michael, Oskam, C., Allentoft, M.
Other Authors:	Shapiro
Format:	Book Chapter
Published:	Humana Press 2012
Subjects:	qPCR standard Library quantitation PCR inhibition Ancient DNA DNA extraction optimization qPCR SYBR Green
Online Access:	http://hdl.handle.net/20.500.11937/3212

Similar Items

Identification of stable reference genes for quantitative PCR in koalas
by: Sarker, N., et al.
Published: (2018)

Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus
by: Rozado-Aguirre, Zuriñe, et al.
Published: (2016)

Molecular methods for the verification of bacterial decontamination
by: Willoughby, Sarah L.
Published: (2021)

Virus deep sequencing: understanding the unknown
by: Rozado Aguirre, Zurine
Published: (2018)

No evidence of local adaptation of immune responses to Gyrodactylus in three-spined stickleback (Gasterosteus aculeatus)
by: Robertson, Shaun, et al.
Published: (2016)

Long-term CO₂ injection and its impact on near-surface soil microbiology
by: Gwosdz, Simone, et al.
Published: (2016)

Toll-like receptors (TLRs) and T cell effector function
by: Mahmood, Majid Mohammed
Published: (2020)

Investigating radiation responsiveness in rectal cancer
by: Hassall, James
Published: (2021)

Metabolic investigation and activity of Cordyceps militaris and cordycepin in cancer cell lines
by: Hafeez, Abdul
Published: (2023)

The application of eDNA for monitoring of the great crested newt in the UK
by: Rees, Helen C., et al.
Published: (2014)

Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets
by: Spiliotopoulos, Anastasios, et al.
Published: (2015)

Molecular biology approaches to quantify subcellular localisation of mRNA therapeutics
by: Smart, Alfredo D.
Published: (2025)

Differentiation of Staphylococcus aureus and Staphylococcus epidermidis by PCR for the fibrinogen binding protein gene.
by: Babra, Charlene, et al.
Published: (2013)

Clinical Evaluation of a MassCode PCR Assay for the Syndromic Detection of Pathogens that cause Respiratory Disease
by: Smith, D., et al.
Published: (2011)

An investigation of metastatic colorectal cancer
by: Asiri, Abutaleb
Published: (2018)

Expression analysis in self-compatible Petunia hybrida and promoter analysis of S-locus F-box genes/SLFs in Petunia inflata
by: Liu, Chen
Published: (2021)

The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology
by: Rees, Helen C., et al.
Published: (2014)

Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells
by: He, J.Q., et al.
Published: (2008)

High-precision dating and ancient DNA profiling of moa (Aves: Dinornithiformes) eggshell documents a complex feature at Wairau Bar and refines the chronology of New Zealand settlement by Polynesians
by: Jacomb, C., et al.
Published: (2014)

Species-level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization
by: Jeunen, G.J., et al.
Published: (2019)

Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections
by: Alfaro-Núñez, A., et al.
Published: (2014)

Microbial community dynamics of a blue-veined raw milk cheese from the UK
by: Yunita, Dewi, et al.
Published: (2018)

Fossil avian eggshell preserves ancient DNA
by: Oskam, C., et al.
Published: (2010)

Molecular and morphological analyses of avian eggshell excavated from a late thirteenth century earth oven
by: Oskam, C., et al.
Published: (2011)

Potency and species specificity of aryl hydrocarbon receptor ligands
by: Wall, Richard John
Published: (2012)

The half-life of DNA in bone: measuring decay kinetics in 158 dated fossils
by: Allentoft, M., et al.
Published: (2012)

Molecular- and pollen-based vegetation analysis in lake sediments from central Scandinavia
by: Parducci, L., et al.
Published: (2013)

Merging ancient and modern DNA: extinct seabird taxon rediscovered in the North Tasman Sea
by: Steeves, T., et al.
Published: (2010)

Analysis of catalytic and non-catalytic regions of the human DNA repair helicase HelQ
by: Jenkins, T
Published: (2021)

Ancient DNA analyses of early archaeological sites in New Zealand reveal extreme exploitation of moa (Aves: Dinornithiformes) at all life stages
by: Oskam, C., et al.
Published: (2012)

Isolation and evolutionary analysis of Australasian topotype of bluetongue virus serotype 4 from India
by: Reddy, Y. Vishnuvardhan, et al.
Published: (2017)

The podosphaera fusca TUB2 gene, a molecular "swiss army knife" with multiple applications in powdery mildew research
by: Vela-Corcía, David, et al.
Published: (2014)

Crevice corrosion of duplex stainless steels in the presence of natural marine biofilms
by: Machuca, Laura, et al.
Published: (2012)

Thorough assessment of DNA preservation from fossil bone and sediments excavated from a late Pleistocenee-Holocene cave deposit on Kangaroo Island, South Australia
by: Haouchar, D., et al.
Published: (2014)

Effects of damping-off caused by Rhizoctonia solani anastomosis group 2-1 on roots of wheat and oil seed rape quantified using X-ray computed tomography and real-time PCR
by: Sturrock, Craig, et al.
Published: (2015)

Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
by: Gerrard, Zara E., et al.
Published: (2018)

Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data
by: Allentoft, M., et al.
Published: (2009)

Mechanisms of Replication Protein A-dependent R-loop resolution by the human DNA repair helicase, HelQ.
by: Betts, Hannah
Published: (2024)

Evidence for a Retroviral Insertion in TRPM1 as the Cause of Congenital Stationary Night Blindness and Leopard Complex Spotting in the Horse
by: Bellone, Rebecca, et al.
Published: (2013)

Xylo-oligosaccharides and inulin affect genotoxicity and bacterial populations differently in a human colonic simulator challenged with soy protein
by: Christophersen, Claus, et al.
Published: (2013)