Quantitative Real-Time PCR in aDNA Research

Quantitative Real-Time PCR in aDNA Research

Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outlin...

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Bibliographic Details
Main Authors: Bunce, Michael, Oskam, C., Allentoft, M.
Other Authors: Shapiro
Format: Book Chapter
Published: Humana Press 2012
Subjects:
qPCR standard
Library quantitation
PCR inhibition
Ancient DNA
DNA extraction optimization
qPCR
SYBR Green
Online Access:http://hdl.handle.net/20.500.11937/3212

Internet

http://hdl.handle.net/20.500.11937/3212

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