Quantitative Real-Time PCR in aDNA Research
Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outlin...
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| Format: | Book Chapter |
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Humana Press
2012
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/3212 |
| _version_ | 1848744169620635648 |
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| author | Bunce, Michael Oskam, C. Allentoft, M. |
| author2 | Shapiro |
| author_facet | Shapiro Bunce, Michael Oskam, C. Allentoft, M. |
| author_sort | Bunce, Michael |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing effi cient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates. |
| first_indexed | 2025-11-14T05:57:12Z |
| format | Book Chapter |
| id | curtin-20.500.11937-3212 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T05:57:12Z |
| publishDate | 2012 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-32122023-02-02T07:57:35Z Quantitative Real-Time PCR in aDNA Research Bunce, Michael Oskam, C. Allentoft, M. Shapiro Beth Hofreiter Michael qPCR standard Library quantitation PCR inhibition Ancient DNA DNA extraction optimization qPCR SYBR Green Quantitative real-time PCR (qPCR) is a technique that is widely used in the fi eld of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing effi cient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates. 2012 Book Chapter http://hdl.handle.net/20.500.11937/3212 10.1007/978-1-61779-516-9_16 Humana Press restricted |
| spellingShingle | qPCR standard Library quantitation PCR inhibition Ancient DNA DNA extraction optimization qPCR SYBR Green Bunce, Michael Oskam, C. Allentoft, M. Quantitative Real-Time PCR in aDNA Research |
| title | Quantitative Real-Time PCR in aDNA Research |
| title_full | Quantitative Real-Time PCR in aDNA Research |
| title_fullStr | Quantitative Real-Time PCR in aDNA Research |
| title_full_unstemmed | Quantitative Real-Time PCR in aDNA Research |
| title_short | Quantitative Real-Time PCR in aDNA Research |
| title_sort | quantitative real-time pcr in adna research |
| topic | qPCR standard Library quantitation PCR inhibition Ancient DNA DNA extraction optimization qPCR SYBR Green |
| url | http://hdl.handle.net/20.500.11937/3212 |