Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens.
This study describes the development of a biodegradable nanoparticulate system for the intranasal delivery of multiple proteins. Chitosan (CS)–dextran sulphate (DS) nanoparticles were developed and optimised for the incorporation of pertussis toxin (PTX) and a potential targeting ligand (immunoglobu...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Informa Healthcare
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/32009 |
| _version_ | 1848753542684213248 |
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| author | Sharma, Sameer Benson, Heather Mukkur, Trilochan Rigby, P. Chen, Y. |
| author_facet | Sharma, Sameer Benson, Heather Mukkur, Trilochan Rigby, P. Chen, Y. |
| author_sort | Sharma, Sameer |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This study describes the development of a biodegradable nanoparticulate system for the intranasal delivery of multiple proteins. Chitosan (CS)–dextran sulphate (DS) nanoparticles were developed and optimised for the incorporation of pertussis toxin (PTX) and a potential targeting ligand (immunoglobulin-A, IgA). In vitro characterization and in vivo uptake studies were performed for the evaluation of developed nanoparticles. The ratio of CS to DS, the order of mixing and pH of nanoparticle suspension were identified as important formulation factors governing the size and zeta potential of nanoparticles. An optimised CS–DS nanoparticle formulation prepared with the CS to DS weight ratio of 3 : 1 was used to load PTX and/or IgA. Entrapment efficiency of >90% was obtained for both. The in vivo uptake of IgA-loaded CS–DS nanoparticles in mice showed a preferential uptake of nanoparticles probably by nasal membranous or microfold cells following intranasal administration. The results of this study indicate the potential application of IgA-loaded CS–DS nanoparticles as a nasal vaccine delivery system. |
| first_indexed | 2025-11-14T08:26:10Z |
| format | Journal Article |
| id | curtin-20.500.11937-32009 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:26:10Z |
| publishDate | 2013 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-320092017-09-13T15:17:40Z Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. Sharma, Sameer Benson, Heather Mukkur, Trilochan Rigby, P. Chen, Y. complex coacervation pertussis toxoid mucosal delivery M cells nasopharynx-associated lymphoid tissue nanoparticles vaccine This study describes the development of a biodegradable nanoparticulate system for the intranasal delivery of multiple proteins. Chitosan (CS)–dextran sulphate (DS) nanoparticles were developed and optimised for the incorporation of pertussis toxin (PTX) and a potential targeting ligand (immunoglobulin-A, IgA). In vitro characterization and in vivo uptake studies were performed for the evaluation of developed nanoparticles. The ratio of CS to DS, the order of mixing and pH of nanoparticle suspension were identified as important formulation factors governing the size and zeta potential of nanoparticles. An optimised CS–DS nanoparticle formulation prepared with the CS to DS weight ratio of 3 : 1 was used to load PTX and/or IgA. Entrapment efficiency of >90% was obtained for both. The in vivo uptake of IgA-loaded CS–DS nanoparticles in mice showed a preferential uptake of nanoparticles probably by nasal membranous or microfold cells following intranasal administration. The results of this study indicate the potential application of IgA-loaded CS–DS nanoparticles as a nasal vaccine delivery system. 2013 Journal Article http://hdl.handle.net/20.500.11937/32009 10.3109/02652048.2012.726279 Informa Healthcare restricted |
| spellingShingle | complex coacervation pertussis toxoid mucosal delivery M cells nasopharynx-associated lymphoid tissue nanoparticles vaccine Sharma, Sameer Benson, Heather Mukkur, Trilochan Rigby, P. Chen, Y. Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| title | Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| title_full | Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| title_fullStr | Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| title_full_unstemmed | Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| title_short | Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| title_sort | preliminary studies on the development of iga-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens. |
| topic | complex coacervation pertussis toxoid mucosal delivery M cells nasopharynx-associated lymphoid tissue nanoparticles vaccine |
| url | http://hdl.handle.net/20.500.11937/32009 |