Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect
The excluded volume effect (EVE) rules all life processes. It is created by macromolecules that occupy a given volume thereby confining other molecules to the remaining space with large consequences on reaction kinetics and molecular assembly. Implementing EVE in fibroblast culture accelerated conve...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Elsevier Science BV
2007
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| Online Access: | http://hdl.handle.net/20.500.11937/31588 |
| _version_ | 1848753421868335104 |
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| author | Lareu, Ricky R. Subramhanya, H. Peng, Y. Benny, P. Chen, C. Wang, Z. Rajagopalan, R. Raghunath, M. |
| author_facet | Lareu, Ricky R. Subramhanya, H. Peng, Y. Benny, P. Chen, C. Wang, Z. Rajagopalan, R. Raghunath, M. |
| author_sort | Lareu, Ricky R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The excluded volume effect (EVE) rules all life processes. It is created by macromolecules that occupy a given volume thereby confining other molecules to the remaining space with large consequences on reaction kinetics and molecular assembly. Implementing EVE in fibroblast culture accelerated conversion of procollagen to collagen by procollagen C-proteinase (PCP/BMP-1) and proteolytic modification of its allosteric regulator, PCOLCE1. This led to a 20–30- and 3–6-fold increased collagen deposition in two- and three-dimensional cultures, respectively, and creation of crosslinked collagen footprints beneath cells. Important parameters correlating with accelerated deposition were hydrodynamic radius of macromolecules and their negative charge density. |
| first_indexed | 2025-11-14T08:24:15Z |
| format | Journal Article |
| id | curtin-20.500.11937-31588 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:24:15Z |
| publishDate | 2007 |
| publisher | Elsevier Science BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-315882017-09-13T15:21:25Z Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect Lareu, Ricky R. Subramhanya, H. Peng, Y. Benny, P. Chen, C. Wang, Z. Rajagopalan, R. Raghunath, M. Macromolecular crowding Extracellular matrix Procollagen C-proteinase Excluded volume effect Collagen deposition The excluded volume effect (EVE) rules all life processes. It is created by macromolecules that occupy a given volume thereby confining other molecules to the remaining space with large consequences on reaction kinetics and molecular assembly. Implementing EVE in fibroblast culture accelerated conversion of procollagen to collagen by procollagen C-proteinase (PCP/BMP-1) and proteolytic modification of its allosteric regulator, PCOLCE1. This led to a 20–30- and 3–6-fold increased collagen deposition in two- and three-dimensional cultures, respectively, and creation of crosslinked collagen footprints beneath cells. Important parameters correlating with accelerated deposition were hydrodynamic radius of macromolecules and their negative charge density. 2007 Journal Article http://hdl.handle.net/20.500.11937/31588 10.1016/j.febslet.2007.05.020 Elsevier Science BV unknown |
| spellingShingle | Macromolecular crowding Extracellular matrix Procollagen C-proteinase Excluded volume effect Collagen deposition Lareu, Ricky R. Subramhanya, H. Peng, Y. Benny, P. Chen, C. Wang, Z. Rajagopalan, R. Raghunath, M. Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect |
| title | Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect |
| title_full | Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect |
| title_fullStr | Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect |
| title_full_unstemmed | Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect |
| title_short | Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect |
| title_sort | collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: the biological relevance of the excluded volume effect |
| topic | Macromolecular crowding Extracellular matrix Procollagen C-proteinase Excluded volume effect Collagen deposition |
| url | http://hdl.handle.net/20.500.11937/31588 |