Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids–in silico predictions and in vitro evidence

Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids–in silico predictions and in vitro evidence

Amplification of DNA in vivo occurs in intracellular environments characterized by macromolecular crowding (MMC). In vitro Polymerase-chain-reaction (PCR), however, is non-crowded, requires thermal cycling for melting of DNA strands, primer-template hybridization and enzymatic primer-extension. The...

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Bibliographic Details
Main Authors: Harve, K., Lareu, Ricky R., Rajagopalan, R., Raghunath, M.
Format: Journal Article
Published: Oxford University Press 2010
Online Access:http://hdl.handle.net/20.500.11937/30252

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