Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids–in silico predictions and in vitro evidence
Amplification of DNA in vivo occurs in intracellular environments characterized by macromolecular crowding (MMC). In vitro Polymerase-chain-reaction (PCR), however, is non-crowded, requires thermal cycling for melting of DNA strands, primer-template hybridization and enzymatic primer-extension. The...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
Oxford University Press
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/30252 |