Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia
The connective tissue growth factor gene (CTGF) is aberrantly expressed in 75% of precursor B-cell acute lymphoblastic leukaemias (pre-B ALL) and is associated with poor outcome. We identified consistent hypomethylation of the CTGF locus in primary pre-B ALL specimens regardless of CTGF expression....
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Published: |
Blackwell Science Ltd
2013
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/28646 |
| _version_ | 1848752592430039040 |
|---|---|
| author | Welch, Mathew Greene, W. Kees, U. |
| author_facet | Welch, Mathew Greene, W. Kees, U. |
| author_sort | Welch, Mathew |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The connective tissue growth factor gene (CTGF) is aberrantly expressed in 75% of precursor B-cell acute lymphoblastic leukaemias (pre-B ALL) and is associated with poor outcome. We identified consistent hypomethylation of the CTGF locus in primary pre-B ALL specimens regardless of CTGF expression. By contrast, primary T-cell ALL specimens, which do not express CTGF, exhibited distinctive patterns of hypermethylation. Furthermore, we confirmed that global changes in DNA methylation and histone acetylation can both functionally modulate CTGF expression in pre-B ALL cell lines. These data suggest that hypomethylation of the CTGF locus is an essential prerequisite for aberrant CTGF expression in pre-B ALL. |
| first_indexed | 2025-11-14T08:11:04Z |
| format | Journal Article |
| id | curtin-20.500.11937-28646 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:11:04Z |
| publishDate | 2013 |
| publisher | Blackwell Science Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-286462017-09-13T15:18:09Z Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia Welch, Mathew Greene, W. Kees, U. bisulfite sequencing pre-B ALL Leukaemia DNA methylation The connective tissue growth factor gene (CTGF) is aberrantly expressed in 75% of precursor B-cell acute lymphoblastic leukaemias (pre-B ALL) and is associated with poor outcome. We identified consistent hypomethylation of the CTGF locus in primary pre-B ALL specimens regardless of CTGF expression. By contrast, primary T-cell ALL specimens, which do not express CTGF, exhibited distinctive patterns of hypermethylation. Furthermore, we confirmed that global changes in DNA methylation and histone acetylation can both functionally modulate CTGF expression in pre-B ALL cell lines. These data suggest that hypomethylation of the CTGF locus is an essential prerequisite for aberrant CTGF expression in pre-B ALL. 2013 Journal Article http://hdl.handle.net/20.500.11937/28646 10.1111/bjh.12417 Blackwell Science Ltd unknown |
| spellingShingle | bisulfite sequencing pre-B ALL Leukaemia DNA methylation Welch, Mathew Greene, W. Kees, U. Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia |
| title | Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia |
| title_full | Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia |
| title_fullStr | Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia |
| title_full_unstemmed | Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia |
| title_short | Hypomethylation of the CTGF gene locus is a common feature of paediatric pre-B acute lymphoblastic leukaemia |
| title_sort | hypomethylation of the ctgf gene locus is a common feature of paediatric pre-b acute lymphoblastic leukaemia |
| topic | bisulfite sequencing pre-B ALL Leukaemia DNA methylation |
| url | http://hdl.handle.net/20.500.11937/28646 |