Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation
The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic a...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/27569 |
| _version_ | 1848752300221267968 |
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| author | Hatanaka, Elaine Dermargos, Alexandre Hirata, Aparecida Vinolo, Marco Carpinelli, Angelo Newsholme, Philip Armelin, Hugo Curi, Rui |
| author_facet | Hatanaka, Elaine Dermargos, Alexandre Hirata, Aparecida Vinolo, Marco Carpinelli, Angelo Newsholme, Philip Armelin, Hugo Curi, Rui |
| author_sort | Hatanaka, Elaine |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. |
| first_indexed | 2025-11-14T08:06:26Z |
| format | Journal Article |
| id | curtin-20.500.11937-27569 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:06:26Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-275692017-09-13T15:06:35Z Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation Hatanaka, Elaine Dermargos, Alexandre Hirata, Aparecida Vinolo, Marco Carpinelli, Angelo Newsholme, Philip Armelin, Hugo Curi, Rui The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. 2013 Journal Article http://hdl.handle.net/20.500.11937/27569 10.1371/journal.pone.0058626 Public Library of Science fulltext |
| spellingShingle | Hatanaka, Elaine Dermargos, Alexandre Hirata, Aparecida Vinolo, Marco Carpinelli, Angelo Newsholme, Philip Armelin, Hugo Curi, Rui Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation |
| title | Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation |
| title_full | Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation |
| title_fullStr | Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation |
| title_full_unstemmed | Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation |
| title_short | Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation |
| title_sort | oleic, linoleic and linolenic acids increase ros production by fibroblasts via nadph oxidase activation |
| url | http://hdl.handle.net/20.500.11937/27569 |