A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses
A number of treatment options are available to multiple sclerosis patients, however this needs to be improved. Herein, we designed and synthesized a number of peptides by mutating principal TCR contact residues based on MBP83-99 peptide epitope. Immunization of SJL/J mice with MBP83-99 and mutant [A...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Elsevier BV
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/26942 |
| _version_ | 1848752127425380352 |
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| author | Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. |
| author_facet | Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. |
| author_sort | Katsara, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A number of treatment options are available to multiple sclerosis patients, however this needs to be improved. Herein, we designed and synthesized a number of peptides by mutating principal TCR contact residues based on MBP83-99 peptide epitope. Immunization of SJL/J mice with MBP83-99 and mutant [A91]MBP83-99, [E91]MBP83-99, [F91]MBP83-99, [Y91]MBP83-99, and [R91, A96]MBP83-99 peptides, induced IFN-?, and only [R91, A96]MBP83-99 mutant peptide was able to induce IL-4 secretion by T cells. T cells against the native MBP83-99 peptide cross-reacted with all peptides except [Y91]MBP83-99 and [R91,A96]MBP83-99. The double mutant [R91, A96]MBP83-99 was able to antagonize IFN-? production in vitro by T cells against the native MBP83-99 peptide. Antibodies generated to [R91, A96]MBP83-99 did not cross-react with whole MBP protein. Molecular modeling between peptide analogs and H2 I-As demonstrated novel interactions. The [R91, A96]MBP83-99 double mutant peptide analog is the most promising for further therapeutic studies. © 2008 Elsevier B.V. All rights reserved. |
| first_indexed | 2025-11-14T08:03:41Z |
| format | Journal Article |
| id | curtin-20.500.11937-26942 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:03:41Z |
| publishDate | 2008 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-269422017-09-13T15:29:11Z A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. A number of treatment options are available to multiple sclerosis patients, however this needs to be improved. Herein, we designed and synthesized a number of peptides by mutating principal TCR contact residues based on MBP83-99 peptide epitope. Immunization of SJL/J mice with MBP83-99 and mutant [A91]MBP83-99, [E91]MBP83-99, [F91]MBP83-99, [Y91]MBP83-99, and [R91, A96]MBP83-99 peptides, induced IFN-?, and only [R91, A96]MBP83-99 mutant peptide was able to induce IL-4 secretion by T cells. T cells against the native MBP83-99 peptide cross-reacted with all peptides except [Y91]MBP83-99 and [R91,A96]MBP83-99. The double mutant [R91, A96]MBP83-99 was able to antagonize IFN-? production in vitro by T cells against the native MBP83-99 peptide. Antibodies generated to [R91, A96]MBP83-99 did not cross-react with whole MBP protein. Molecular modeling between peptide analogs and H2 I-As demonstrated novel interactions. The [R91, A96]MBP83-99 double mutant peptide analog is the most promising for further therapeutic studies. © 2008 Elsevier B.V. All rights reserved. 2008 Journal Article http://hdl.handle.net/20.500.11937/26942 10.1016/j.jneuroim.2008.06.013 Elsevier BV restricted |
| spellingShingle | Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses |
| title | A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses |
| title_full | A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses |
| title_fullStr | A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses |
| title_full_unstemmed | A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses |
| title_short | A double mutation of MBP83-99 peptide induces IL-4 responses and antagonizes IFN-? responses |
| title_sort | double mutation of mbp83-99 peptide induces il-4 responses and antagonizes ifn-? responses |
| url | http://hdl.handle.net/20.500.11937/26942 |