Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats
The aim is to investigate the influence of the antidiabetic drug gliclazide on the ileal permeation of the semisynthetic bile acid, MKC, in tissues from healthy and diabetic rats. Sixteen Wistar rats (350 +/- 50 g) were randomly allocated into four groups (4 rats per group, 8 chambers per rat, i.e.,...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Springer France
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/25466 |
| _version_ | 1848751717200429056 |
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| author | Al-Salami, Hani Butt, G. Tucker, I. Fawcett, P. Golocorbin-Kon, S. Mikov, I. Mikov, M. |
| author_facet | Al-Salami, Hani Butt, G. Tucker, I. Fawcett, P. Golocorbin-Kon, S. Mikov, I. Mikov, M. |
| author_sort | Al-Salami, Hani |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The aim is to investigate the influence of the antidiabetic drug gliclazide on the ileal permeation of the semisynthetic bile acid, MKC, in tissues from healthy and diabetic rats. Sixteen Wistar rats (350 +/- 50 g) were randomly allocated into four groups (4 rats per group, 8 chambers per rat, i.e., n=32) two of which were made diabetic (given alloxan i.v. 30 mg/kg). Group 1 was used to measure the permeation of MKC (50 microg/ml) alone (control) while group 2 to measure MKC permeation in the presence of gliclazide (200 microg/ml). The diabetic groups 3 (gliclazide) and 4 (MKC+gliclazide) were treated in the same way. Rats were sacrificed and tissues were mounted into the Ussing chamber for the measurement of MKC mucosal to serosal (absorptive) and serosal to mucosal (secretory) fluxes. In healthy tissues, gliclazide reduced MKC absorptive flux (p < 0.01) and increased its secretory flux (p < 0.01). In diabetic tissues, gliclazide had no effect on either the absorptive or the secretory fluxes of MKC. The lack of effect of gliclazide on MKC permeation in diabetic tissues suggests the absence or suppressed drug transporters. Furthermore, gliclazide inhibition of MKC absorptive flux and induction of MKC secretory flux in healthy tissues may result from the selective inhibition of an efflux drug transporter. |
| first_indexed | 2025-11-14T07:57:10Z |
| format | Journal Article |
| id | curtin-20.500.11937-25466 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:57:10Z |
| publishDate | 2009 |
| publisher | Springer France |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-254662017-01-30T12:48:38Z Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats Al-Salami, Hani Butt, G. Tucker, I. Fawcett, P. Golocorbin-Kon, S. Mikov, I. Mikov, M. permeation Mrp3 transporters gliclazide diabetes bile acid The aim is to investigate the influence of the antidiabetic drug gliclazide on the ileal permeation of the semisynthetic bile acid, MKC, in tissues from healthy and diabetic rats. Sixteen Wistar rats (350 +/- 50 g) were randomly allocated into four groups (4 rats per group, 8 chambers per rat, i.e., n=32) two of which were made diabetic (given alloxan i.v. 30 mg/kg). Group 1 was used to measure the permeation of MKC (50 microg/ml) alone (control) while group 2 to measure MKC permeation in the presence of gliclazide (200 microg/ml). The diabetic groups 3 (gliclazide) and 4 (MKC+gliclazide) were treated in the same way. Rats were sacrificed and tissues were mounted into the Ussing chamber for the measurement of MKC mucosal to serosal (absorptive) and serosal to mucosal (secretory) fluxes. In healthy tissues, gliclazide reduced MKC absorptive flux (p < 0.01) and increased its secretory flux (p < 0.01). In diabetic tissues, gliclazide had no effect on either the absorptive or the secretory fluxes of MKC. The lack of effect of gliclazide on MKC permeation in diabetic tissues suggests the absence or suppressed drug transporters. Furthermore, gliclazide inhibition of MKC absorptive flux and induction of MKC secretory flux in healthy tissues may result from the selective inhibition of an efflux drug transporter. 2009 Journal Article http://hdl.handle.net/20.500.11937/25466 Springer France restricted |
| spellingShingle | permeation Mrp3 transporters gliclazide diabetes bile acid Al-Salami, Hani Butt, G. Tucker, I. Fawcett, P. Golocorbin-Kon, S. Mikov, I. Mikov, M. Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats |
| title | Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats |
| title_full | Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats |
| title_fullStr | Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats |
| title_full_unstemmed | Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats |
| title_short | Gliclazide reduces MKC intestinal transport in healthy but not diabetic rats |
| title_sort | gliclazide reduces mkc intestinal transport in healthy but not diabetic rats |
| topic | permeation Mrp3 transporters gliclazide diabetes bile acid |
| url | http://hdl.handle.net/20.500.11937/25466 |