The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line

The Wnt signalling pathway is involved in regulating cellular proliferation and differentiation, and aberrant activation has been described in several cancers including breast. Oestradiol up regulates Wnt pathway gene expression, and thereby activates the Wnt signalling pathway. We used the oestroge...

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Main Authors: McLaren, S., Arfuso, Frank, Zeps, N., Dharmarajan, Arunasalam
Format: Journal Article
Published: Lifescience Global 2014
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/20199
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author McLaren, S.
Arfuso, Frank
Zeps, N.
Dharmarajan, Arunasalam
author_facet McLaren, S.
Arfuso, Frank
Zeps, N.
Dharmarajan, Arunasalam
author_sort McLaren, S.
building Curtin Institutional Repository
collection Online Access
description The Wnt signalling pathway is involved in regulating cellular proliferation and differentiation, and aberrant activation has been described in several cancers including breast. Oestradiol up regulates Wnt pathway gene expression, and thereby activates the Wnt signalling pathway. We used the oestrogen-responsive breast cancer cell line MCF-7 to examine the effects of secreted frizzled related protein 4 (sFRP-4) on oestradiol-induced growth, including gene expression of the Wnt signalling pathway genes Frizzled Receptor, Wnt-10b, and β-catenin. We demonstrate here that sFRP-4 inhibits oestradiol-induced cell growth in the MCF-7 cell line and also down regulates oestradiol-induced expression of selected Wnt signalling genes including β-catenin. We propose that sFRP-4 is a potent inhibitor of the Wnt signalling pathway and may negatively regulate oestradiol-mediated proliferation in human breast cancer cells.
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spelling curtin-20.500.11937-201992017-09-13T13:49:38Z The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line McLaren, S. Arfuso, Frank Zeps, N. Dharmarajan, Arunasalam β-catenin cellular proliferation Wnt signalling oestradiol sFRP4 Breast cancer growth inhibition The Wnt signalling pathway is involved in regulating cellular proliferation and differentiation, and aberrant activation has been described in several cancers including breast. Oestradiol up regulates Wnt pathway gene expression, and thereby activates the Wnt signalling pathway. We used the oestrogen-responsive breast cancer cell line MCF-7 to examine the effects of secreted frizzled related protein 4 (sFRP-4) on oestradiol-induced growth, including gene expression of the Wnt signalling pathway genes Frizzled Receptor, Wnt-10b, and β-catenin. We demonstrate here that sFRP-4 inhibits oestradiol-induced cell growth in the MCF-7 cell line and also down regulates oestradiol-induced expression of selected Wnt signalling genes including β-catenin. We propose that sFRP-4 is a potent inhibitor of the Wnt signalling pathway and may negatively regulate oestradiol-mediated proliferation in human breast cancer cells. 2014 Journal Article http://hdl.handle.net/20.500.11937/20199 10.6000/1927-7229.2014.03.01.1 Lifescience Global fulltext
spellingShingle β-catenin
cellular proliferation
Wnt signalling
oestradiol
sFRP4
Breast cancer
growth inhibition
McLaren, S.
Arfuso, Frank
Zeps, N.
Dharmarajan, Arunasalam
The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line
title The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line
title_full The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line
title_fullStr The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line
title_full_unstemmed The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line
title_short The Role of Secreted Frizzled Related Protein 4 (sFRP-4) in Regulating Oestradiol-Induced Growth of the MCF-7 Breast Cancer Cell Line
title_sort role of secreted frizzled related protein 4 (sfrp-4) in regulating oestradiol-induced growth of the mcf-7 breast cancer cell line
topic β-catenin
cellular proliferation
Wnt signalling
oestradiol
sFRP4
Breast cancer
growth inhibition
url http://hdl.handle.net/20.500.11937/20199