The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins

This study was conducted to evaluate the effects of protein isolation on dietary fibre and whey proteins of lupin flour. Commercially available flour was used in-order to understand the possible commercial applications of the results generated by the project. Protein isolation process from lupin flo...

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Main Authors: Coorey, Ranil, Chao, K., Kumar, Vivek, Jayasena, Vijay
Format: Journal Article
Published: Wiley-Blackwell Publishers 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/19110
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author Coorey, Ranil
Chao, K.
Kumar, Vivek
Jayasena, Vijay
author_facet Coorey, Ranil
Chao, K.
Kumar, Vivek
Jayasena, Vijay
author_sort Coorey, Ranil
building Curtin Institutional Repository
collection Online Access
description This study was conducted to evaluate the effects of protein isolation on dietary fibre and whey proteins of lupin flour. Commercially available flour was used in-order to understand the possible commercial applications of the results generated by the project. Protein isolation process from lupin flour that was first defatted, resulted in three products: (1) the dietary fibre fraction; (2) the protein isolate; and (3) the soluble lupin whey protein supernatant. The total dietary fibre (TDF), insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) contents in the flour were 28.9, 19.1, and 9.9%, respectively. The TDF, IDF and SDF of the dietary fibre fractions were 72.1, 65.8 and 6.3% respectively. A salting out process for the isolation of the whey protein fraction with different salts including trichloroacetic acid was evaluated. Emulsification and foaming properties of the whey proteins were significantly affected by the type of salt. The functional properties of the control whey protein isolated by a simple additional centrifugation step were significantly better than those isolated with any of the salts. The results showed that the lupin whey protein with good functional properties can be produced from the whey fraction.
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format Journal Article
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institution Curtin University Malaysia
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publishDate 2013
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spelling curtin-20.500.11937-191102017-09-13T15:43:05Z The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins Coorey, Ranil Chao, K. Kumar, Vivek Jayasena, Vijay soluble dietary fibre insoluble dietary fibre total dietary fibre protein extraction lupin This study was conducted to evaluate the effects of protein isolation on dietary fibre and whey proteins of lupin flour. Commercially available flour was used in-order to understand the possible commercial applications of the results generated by the project. Protein isolation process from lupin flour that was first defatted, resulted in three products: (1) the dietary fibre fraction; (2) the protein isolate; and (3) the soluble lupin whey protein supernatant. The total dietary fibre (TDF), insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) contents in the flour were 28.9, 19.1, and 9.9%, respectively. The TDF, IDF and SDF of the dietary fibre fractions were 72.1, 65.8 and 6.3% respectively. A salting out process for the isolation of the whey protein fraction with different salts including trichloroacetic acid was evaluated. Emulsification and foaming properties of the whey proteins were significantly affected by the type of salt. The functional properties of the control whey protein isolated by a simple additional centrifugation step were significantly better than those isolated with any of the salts. The results showed that the lupin whey protein with good functional properties can be produced from the whey fraction. 2013 Journal Article http://hdl.handle.net/20.500.11937/19110 10.3920/QAS2012.0189 Wiley-Blackwell Publishers restricted
spellingShingle soluble dietary fibre
insoluble dietary fibre
total dietary fibre
protein extraction
lupin
Coorey, Ranil
Chao, K.
Kumar, Vivek
Jayasena, Vijay
The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
title The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
title_full The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
title_fullStr The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
title_full_unstemmed The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
title_short The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
title_sort effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteins
topic soluble dietary fibre
insoluble dietary fibre
total dietary fibre
protein extraction
lupin
url http://hdl.handle.net/20.500.11937/19110