Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model
Background: Pigment epithelium-derived factor (PEDF) is an endogenous glycoprotein with a potential role as a therapeutic for osteosarcoma. Animal studies have demonstrated the biological effects of PEDF on osteosarcoma; however, these results are difficult to extrapolate for human use due to the ch...
| Main Authors: | , , |
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| Format: | Journal Article |
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Nature Publishing Group
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/19073 |
| _version_ | 1848749928944238592 |
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| author | Broadhead, M. Dass, Crispin Choong, P. |
| author_facet | Broadhead, M. Dass, Crispin Choong, P. |
| author_sort | Broadhead, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Background: Pigment epithelium-derived factor (PEDF) is an endogenous glycoprotein with a potential role as a therapeutic for osteosarcoma. Animal studies have demonstrated the biological effects of PEDF on osteosarcoma; however, these results are difficult to extrapolate for human use due to the chosen study design and drug delivery methods. Methods: In this study we have attempted to replicate the human presentation and treatment of osteosarcoma using a murine orthotopic model of osteosarcoma. The effects of PEDF on osteosarcoma cell lines were evaluated in vitro prior to animal experimentation. Orthotopic tumours were induced by intra-tibial injection of SaOS-2 osteosarcoma cells. Treatment with PEDF was delayed until after the macroscopic appearance of primary tumours. Pigment epithelium-derived factor was administered systemically via an implanted intraperitoneal micro-osmotic pump. Results: In vitro, PEDF inhibited proliferation, induced apoptosis and inhibited cell cycling of osteosarcoma cells. Pigment epithelium-derived factor promoted adhesion to Collagen I and inhibited invasion through Collagen I. In vivo, treatment with PEDF caused a reduction in both primary tumour volume and burden of pulmonary metastases. Systemic administration of PEDF did not cause toxic effects on normal tissues. Conclusion: Systemically delivered PEDF is effective in suppressing the size of primary and secondary tumours in an orthotopic murine model of osteosarcoma. |
| first_indexed | 2025-11-14T07:28:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-19073 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:28:44Z |
| publishDate | 2011 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-190732017-09-13T13:46:48Z Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model Broadhead, M. Dass, Crispin Choong, P. Background: Pigment epithelium-derived factor (PEDF) is an endogenous glycoprotein with a potential role as a therapeutic for osteosarcoma. Animal studies have demonstrated the biological effects of PEDF on osteosarcoma; however, these results are difficult to extrapolate for human use due to the chosen study design and drug delivery methods. Methods: In this study we have attempted to replicate the human presentation and treatment of osteosarcoma using a murine orthotopic model of osteosarcoma. The effects of PEDF on osteosarcoma cell lines were evaluated in vitro prior to animal experimentation. Orthotopic tumours were induced by intra-tibial injection of SaOS-2 osteosarcoma cells. Treatment with PEDF was delayed until after the macroscopic appearance of primary tumours. Pigment epithelium-derived factor was administered systemically via an implanted intraperitoneal micro-osmotic pump. Results: In vitro, PEDF inhibited proliferation, induced apoptosis and inhibited cell cycling of osteosarcoma cells. Pigment epithelium-derived factor promoted adhesion to Collagen I and inhibited invasion through Collagen I. In vivo, treatment with PEDF caused a reduction in both primary tumour volume and burden of pulmonary metastases. Systemic administration of PEDF did not cause toxic effects on normal tissues. Conclusion: Systemically delivered PEDF is effective in suppressing the size of primary and secondary tumours in an orthotopic murine model of osteosarcoma. 2011 Journal Article http://hdl.handle.net/20.500.11937/19073 10.1038/bjc.2011.410 Nature Publishing Group unknown |
| spellingShingle | Broadhead, M. Dass, Crispin Choong, P. Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model |
| title | Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model |
| title_full | Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model |
| title_fullStr | Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model |
| title_full_unstemmed | Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model |
| title_short | Systemically administered PEDF against primary and secondary tumours in a clinically relevant osteosarcoma model |
| title_sort | systemically administered pedf against primary and secondary tumours in a clinically relevant osteosarcoma model |
| url | http://hdl.handle.net/20.500.11937/19073 |