Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor

The amyloid- (A) peptide is neurotoxic and associated with the pathology of Alzheimer's disease (AD). We investigated the effect of A peptides on insulin binding to the insulin receptor because it is known that (1) A and insulin are both amyloidogenic peptides sharing a common sequence recognit...

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Main Authors: Xie, Ling, Helmerhorst, Erik, Plewright, Brian, Van Bronswijk, Wilhelm, Martins, R.
Format: Journal Article
Published: Society of Neuroscience 2002
Subjects:
Online Access:http://www.jneurosci.org/content/vol22/issue10/
http://hdl.handle.net/20.500.11937/19003
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author Xie, Ling
Helmerhorst, Erik
Plewright, Brian
Van Bronswijk, Wilhelm
Martins, R.
author_facet Xie, Ling
Helmerhorst, Erik
Plewright, Brian
Van Bronswijk, Wilhelm
Martins, R.
author_sort Xie, Ling
building Curtin Institutional Repository
collection Online Access
description The amyloid- (A) peptide is neurotoxic and associated with the pathology of Alzheimer's disease (AD). We investigated the effect of A peptides on insulin binding to the insulin receptor because it is known that (1) A and insulin are both amyloidogenic peptides sharing a common sequence recognition motif, (2) A and insulin are substrates for the same insulin degrading enzyme, and (3) impaired glucose metabolism is a characteristic event in the pathology of AD. We discovered that A1-40 and A1-42, the main physiological forms, reduced insulin binding and receptor autophosphorylation. The reduction in binding was caused by a decrease in the affinity of insulin binding to the insulin receptor. This reduction was independent of the receptor concentration. The reverse, control peptide A40-1 did not reduce insulin binding or insulin receptor autophosphorylation. These results demonstrate that A is a direct competitive inhibitor of insulin binding and action. We speculate that the increased levels of A in Alzheimer's disease may be linked to the associated insulin resistance that has been observed previously in this disease.
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spelling curtin-20.500.11937-190032017-08-23T07:51:42Z Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor Xie, Ling Helmerhorst, Erik Plewright, Brian Van Bronswijk, Wilhelm Martins, R. insulin binding Scatchard analysis diabetes Alzheimer's disease insulin receptors amyloid- peptide The amyloid- (A) peptide is neurotoxic and associated with the pathology of Alzheimer's disease (AD). We investigated the effect of A peptides on insulin binding to the insulin receptor because it is known that (1) A and insulin are both amyloidogenic peptides sharing a common sequence recognition motif, (2) A and insulin are substrates for the same insulin degrading enzyme, and (3) impaired glucose metabolism is a characteristic event in the pathology of AD. We discovered that A1-40 and A1-42, the main physiological forms, reduced insulin binding and receptor autophosphorylation. The reduction in binding was caused by a decrease in the affinity of insulin binding to the insulin receptor. This reduction was independent of the receptor concentration. The reverse, control peptide A40-1 did not reduce insulin binding or insulin receptor autophosphorylation. These results demonstrate that A is a direct competitive inhibitor of insulin binding and action. We speculate that the increased levels of A in Alzheimer's disease may be linked to the associated insulin resistance that has been observed previously in this disease. 2002 Journal Article http://hdl.handle.net/20.500.11937/19003 http://www.jneurosci.org/content/vol22/issue10/ Society of Neuroscience fulltext
spellingShingle insulin binding
Scatchard analysis
diabetes
Alzheimer's disease
insulin receptors
amyloid- peptide
Xie, Ling
Helmerhorst, Erik
Plewright, Brian
Van Bronswijk, Wilhelm
Martins, R.
Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
title Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
title_full Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
title_fullStr Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
title_full_unstemmed Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
title_short Alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
title_sort alzheimer's beta-amyloid peptides compete for insulin binding to the insulin receptor
topic insulin binding
Scatchard analysis
diabetes
Alzheimer's disease
insulin receptors
amyloid- peptide
url http://www.jneurosci.org/content/vol22/issue10/
http://hdl.handle.net/20.500.11937/19003