Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions

QR Code

Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions

Collagen contains the unique imino acid hydroxyproline (HyPro), which is involved in the stabilization of this triple helical molecule. The concentration of HyPro is customarily used to calculate the total collagen content in a cell culture environment and in acid hydrolysates of normal and pathophy...

Full description

Bibliographic Details
Main Authors:	Lareu, Ricky R., Zeugolis, D., Abu-Rub, M., Pandit, A., Raghunath, M.
Format:	Journal Article
Published:	Elsevier BV 2010
Subjects:	Ultrafiltration Hydroxyproline assay Collagen content Sircol assay Sirius red
Online Access:	http://hdl.handle.net/20.500.11937/18496

Similar Items

Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note
by: Zeugolis, D., et al.
Published: (2008)

Controlling equine influenza: traditional to next generation serological assays
by: Kinsley, Rebecca, et al.
Published: (2016)

Investigation into the effect of formulation on intravenous lipid emulsion metabolism using a novel in vitro fluorescent assay
by: Dougall, Paul W.R.
Published: (2016)

Establishment and validation of a monoclonal cell-based reporter assay for screening of hypoxia-inducible factor activities
by: Liew, Sien Yei
Published: (2017)

Characterising Fish Collagen for Expression in Bacterial Systems
by: Shi, Xiaoqin
Published: (2025)

Extraction and characterization of acetic acid-soluble collagen and papain-soluble collagen hydrolysate from poultry eggshell membrane
by: Ma, Roazita
Published: (2016)

Extraction of collagen from fish waste and determination of its physico-chemical characteristics / Nurul Asyiraf Abdul Jabar
by: Abdul Jabar, Nurul Asyiraf
Published: (2011)

Detection of Bifidobacteria Breve by Indirect Enzymelinked Immunosorbent Assay (Elisa)
by: Tee, Siew Choon
Published: (2001)

The application of protein microarray assays in psychoneuroimmunology
by: Ayling, K., et al.
Published: (2017)

Redesign of collagen casings for high quality performance using food grade polysaccharides
by: Sobanwa, Motolani
Published: (2022)

Development of fluorescent nanosensors for real-time biological assays
by: Elsutohy, Mohamed
Published: (2016)

Extraction of collagen from catfish (Clarias gariepnus) waste and determination of its physico-chemical properties / Normah Ismail and Nurul Asyiraf Abdul Jabar
by: Ismail, Normah, et al.
Published: (2012)

DEVELOPING BACTERIOPHAGE-BASED ASSAYS FOR THE RAPID DETECTION OF MYCOBACTERIA
by: Handley-Hartill, Wade
Published: (2023)

Identifying collagen bundles in the most superficial layer of normal articular cartilage
by: Duan, X., et al.
Published: (2009)

Bioassay-Guided Fractionation of the Active Constituent of Juniperus Chinensis
by: Ismail, Intan Safinar
Published: (1997)

Development of a toxicity bioassay system using Photobacterium sp. strain mie
by: Halmi, Mohd Izuan Effendi
Published: (2013)

Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect
by: Lareu, Ricky R., et al.
Published: (2007)

Development and evaluation of enzyme-linked immunosorbent assays for the detection of gelatin sources
by: Tukiran, Nur Azira
Published: (2016)

Development and validation of a mass spectrometry-based assay for quantification of insulin-like factor 3 in human serum
by: Anand-Ivell, Ravinder, et al.
Published: (2018)

Characterization and physicochemical properties of collagen extracted from barramundi (Lates calcarifer, block) skin
by: Mohamad Razali, Umi Hartina
Published: (2012)

Longitudinal change in collagen degradation biomarkers in idiopathic pulmonary fibrosis: an analysis from the prospective, multicentre PROFILE study
by: Jenkins, R. Gisli, et al.
Published: (2015)

Next generation sequencing: validation of S5 GeneStudio Prime and toxicity assay
by: Collier, Thomas
Published: (2021)

Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria
by: Swift, Benjamin, et al.
Published: (2014)

Urine protein concentration estimation for biomarker discovery
by: Mistry, Hiten D., et al.
Published: (2013)

Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
by: Mohamed Mackeen, Muhammad Mukram
Published: (1998)

Development of a microbial bioassay system for detection of boric acid using Paecilomyces variotii.
by: Ang, Swi See
Published: (2011)

Bioassay-guided fractionation of anticoagulant compounds from Melastoma malabathricum Linn. Leaf
by: Khoo, Li Teng
Published: (2015)

Design, synthesis, and evaluation of inhibitors of InhA to prevent cell wall biosynthesis in Mycobacterium bovis
by: Stevenson, Matthew
Published: (2020)

Detection of Beta-Agonist Residues in Meat Using Enzyme Linked Immunosorbent Assay and Gas Chromatography Mass Spectrometry
by: S. Ponniah, P. Jeyaletchumi
Published: (2003)

The optimisation of Pseudotyped viruses for the characterisation of immune responses to equine influenza virus
by: Scott, Simon D., et al.
Published: (2016)

Bioassay-guided identification of anti-cholinesterase and ichthyotoxic compounds from Walsura chrysogyne (Miq.) Bakh.f.apud van steenis
by: Mahmod, Ilya Iryani
Published: (2012)

Collagen modified porous pHEMA–TiO2 composite hydrogels for tissue engineering
by: Li, Chao, et al.
Published: (2013)

Production and characterization of collagen and gelatin from edible jellyfish (Acromitus hardenbergi Stiasny, 1934) for fabrication of biofunctional scaffold
by: Khong, Nicholas Mun Hoe
Published: (2014)

Pigment epithelium-derived factor upregulates collagen I and downregulates matrix metalloproteinase 2 in osteosarcoma cells, and colocalises to collagen I and heat shock protein 47 in fetal and adult bone
by: Alcantara, M., et al.
Published: (2014)

Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro
by: Ortega-Rivas, Antonio, et al.
Published: (2016)

Development and evaluation of recombinant outer membrane protein i-elisa for diagnosis of caprine brucellosis
by: Ahmed, Ihsan Muneer
Published: (2013)

Texture analysis of the 3D collagen network and automatic classification of the physiology of articular cartilage
by: Duan, X., et al.
Published: (2014)

The Scar-in-a-Jar: studying potential anti?brotic compounds from the epigenetic to extracellular level in a single well
by: Chen, C., et al.
Published: (2009)

Microstructural analysis of collagen and elastin fibres in the kangaroo articular cartilage reveals a structural divergence depending on its local mechanical environment
by: He, Bo, et al.
Published: (2013)

Development Of An Indirect Elisa Using The Recombinant Nucleocapsid Protein As Antigen For Detection Of Antibodies To Newcastle Disease Virus
by: Md Saad, Norsharina
Published: (2007)

Cannot write session to /tmp/vufind_sessions/sess_4m02tr9aamdhtg6qgmc5j1n73k