Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions

Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions

Collagen contains the unique imino acid hydroxyproline (HyPro), which is involved in the stabilization of this triple helical molecule. The concentration of HyPro is customarily used to calculate the total collagen content in a cell culture environment and in acid hydrolysates of normal and pathophy...

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Bibliographic Details
Main Authors: Lareu, Ricky R., Zeugolis, D., Abu-Rub, M., Pandit, A., Raghunath, M.
Format: Journal Article
Published: Elsevier BV 2010
Subjects:
Ultrafiltration
Hydroxyproline assay
Collagen content
Sircol assay
Sirius red
Online Access:http://hdl.handle.net/20.500.11937/18496

Internet

http://hdl.handle.net/20.500.11937/18496

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