Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transfo...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
1987
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| Online Access: | http://hdl.handle.net/20.500.11937/14903 |
| _version_ | 1848748747464376320 |
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| author | Punt, P. Oliver, Richard Dingemanse, M. Pouwels, P. Van Den Hondel, C. |
| author_facet | Punt, P. Oliver, Richard Dingemanse, M. Pouwels, P. Van Den Hondel, C. |
| author_sort | Punt, P. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transformation vector was constructed which contains this marker and confers HmB resistance to Aspergillus species.With both A. niger and A. nidulans, transformation frequencies of 5–20 transformants per μg vector DNA were obtained. Cotransformation with other vectors was shown to be very efficient in both species, when selection for HmB resistance was applied. |
| first_indexed | 2025-11-14T07:09:57Z |
| format | Journal Article |
| id | curtin-20.500.11937-14903 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:09:57Z |
| publishDate | 1987 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-149032017-09-13T15:54:08Z Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli Punt, P. Oliver, Richard Dingemanse, M. Pouwels, P. Van Den Hondel, C. A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transformation vector was constructed which contains this marker and confers HmB resistance to Aspergillus species.With both A. niger and A. nidulans, transformation frequencies of 5–20 transformants per μg vector DNA were obtained. Cotransformation with other vectors was shown to be very efficient in both species, when selection for HmB resistance was applied. 1987 Journal Article http://hdl.handle.net/20.500.11937/14903 10.1016/0378-1119(87)90164-8 restricted |
| spellingShingle | Punt, P. Oliver, Richard Dingemanse, M. Pouwels, P. Van Den Hondel, C. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli |
| title | Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli |
| title_full | Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli |
| title_fullStr | Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli |
| title_full_unstemmed | Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli |
| title_short | Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli |
| title_sort | transformation of aspergillus based on the hygromycin b resistance marker from escherichia coli |
| url | http://hdl.handle.net/20.500.11937/14903 |