Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers
Platinum compounds such as cisplatin and carboplatin are frequently used as the first-line chemotherapy for the treatment of the head and neck squamous cell carcinoma (HNSCC). In the present study, we investigated whether garcinol, a polyisoprenylated benzophenone can chemosensitize HNSCC to cisplat...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Impact Journals LLC
2015
|
| Online Access: | http://hdl.handle.net/20.500.11937/14687 |
| _version_ | 1848748689717198848 |
|---|---|
| author | Li, F. Shanmugam, M. Siveen, K. Wang, F. Ong, T. Loo, S. Swamy, M. Mandal, S. Kumar, Alan Prem Goh, B. Kundu, T. Ahn, K. Wang, L. Hui, K. Sethi, Gautam |
| author_facet | Li, F. Shanmugam, M. Siveen, K. Wang, F. Ong, T. Loo, S. Swamy, M. Mandal, S. Kumar, Alan Prem Goh, B. Kundu, T. Ahn, K. Wang, L. Hui, K. Sethi, Gautam |
| author_sort | Li, F. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Platinum compounds such as cisplatin and carboplatin are frequently used as the first-line chemotherapy for the treatment of the head and neck squamous cell carcinoma (HNSCC). In the present study, we investigated whether garcinol, a polyisoprenylated benzophenone can chemosensitize HNSCC to cisplatin. We found that garcinol inhibited the viability of a panel of diverse HNSCC cell lines, enhanced the apoptotic effect of cisplatin, suppressed constitutive as well as cisplatin-induced NF-?B activation, and downregulated the expression of various oncogenic gene products (cyclin D1, Bcl-2, survivin and VEGF). In vivo study showed that administration of garcinol alone (0.5 mg/kg body weight, i.p. five times/week) significantly suppressed the growth of the tumor, and this effect was further increased by cisplatin. Both the markers of proliferation index (Ki-67) and microvessel density (CD31) were downregulated in tumor tissues by the combination of cisplatin and garcinol. The pharmacokinetic results of garcinol indicated that good systemic exposure was achievable after i.p. administration of garcinol at 0.5 mg/kg and 2 mg/kg with mean peak concentration (Cmax) of 1825.4 and 6635.7 nM in the mouse serum, respectively. Overall, our results suggest that garcinol can indeed potentiate the effects of cisplatin by negative regulation of various inflammatory and proliferative biomarkers. |
| first_indexed | 2025-11-14T07:09:02Z |
| format | Journal Article |
| id | curtin-20.500.11937-14687 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:09:02Z |
| publishDate | 2015 |
| publisher | Impact Journals LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-146872017-01-30T11:45:23Z Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers Li, F. Shanmugam, M. Siveen, K. Wang, F. Ong, T. Loo, S. Swamy, M. Mandal, S. Kumar, Alan Prem Goh, B. Kundu, T. Ahn, K. Wang, L. Hui, K. Sethi, Gautam Platinum compounds such as cisplatin and carboplatin are frequently used as the first-line chemotherapy for the treatment of the head and neck squamous cell carcinoma (HNSCC). In the present study, we investigated whether garcinol, a polyisoprenylated benzophenone can chemosensitize HNSCC to cisplatin. We found that garcinol inhibited the viability of a panel of diverse HNSCC cell lines, enhanced the apoptotic effect of cisplatin, suppressed constitutive as well as cisplatin-induced NF-?B activation, and downregulated the expression of various oncogenic gene products (cyclin D1, Bcl-2, survivin and VEGF). In vivo study showed that administration of garcinol alone (0.5 mg/kg body weight, i.p. five times/week) significantly suppressed the growth of the tumor, and this effect was further increased by cisplatin. Both the markers of proliferation index (Ki-67) and microvessel density (CD31) were downregulated in tumor tissues by the combination of cisplatin and garcinol. The pharmacokinetic results of garcinol indicated that good systemic exposure was achievable after i.p. administration of garcinol at 0.5 mg/kg and 2 mg/kg with mean peak concentration (Cmax) of 1825.4 and 6635.7 nM in the mouse serum, respectively. Overall, our results suggest that garcinol can indeed potentiate the effects of cisplatin by negative regulation of various inflammatory and proliferative biomarkers. 2015 Journal Article http://hdl.handle.net/20.500.11937/14687 Impact Journals LLC restricted |
| spellingShingle | Li, F. Shanmugam, M. Siveen, K. Wang, F. Ong, T. Loo, S. Swamy, M. Mandal, S. Kumar, Alan Prem Goh, B. Kundu, T. Ahn, K. Wang, L. Hui, K. Sethi, Gautam Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| title | Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| title_full | Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| title_fullStr | Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| title_full_unstemmed | Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| title_short | Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| title_sort | garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers |
| url | http://hdl.handle.net/20.500.11937/14687 |