Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
A biodegradable scaffold in tissue engineering serves as a temporary skeleton to accommodate and stimulate new tissue growth. Alginate (Alg) and chitosan (Chi) are both popular materials applied as biomaterials or bioimplants. However, Alg derived from brown algae is highly compliant and easily deco...
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| Format: | Thesis |
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2017
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| Online Access: | http://eprints.uthm.edu.my/9919/ http://eprints.uthm.edu.my/9919/1/ROSLINDA_HANI_RAMLI.pdf |
| Summary: | A biodegradable scaffold in tissue engineering serves as a temporary skeleton to
accommodate and stimulate new tissue growth. Alginate (Alg) and chitosan (Chi) are
both popular materials applied as biomaterials or bioimplants. However, Alg derived
from brown algae is highly compliant and easily decomposed in fluid, whilst Chi
derived from shrimp shells has weak strength. In rectify these problems, the
development of Chi and Alg based biodegradable scaffolds incorporated with silver
nanoparticles (AgNPs) with enhanced mechanical properties and biosafe function is
proposed. Different ratios of chitosan/alginate (Chi/Alg) were prepared and the effect
of different ratio (1:1, 1:2 and 2:1) to the mechanical, biological properties with and
without AgNPs and keratinocyte cell growth were investigated. The preliminary
result of FTIR, UV-Vis, XRD, FESEM and EDS proved the production of silver
nanoparticles. Meanwhile, FTIR, swelling/degradation, DMA, TGA, DSC, FESEM
and MTT assay was conducted to study the properties of Chi/Alg based scaffold.
FTIR analysis shows the crosslinking of Chi/Alg based scaffold.
Swelling/degradation and DMA shows Chi/Alg and chitosan/alginate/silver
nanoparticles (Chi/Alg/AgNPs) has adequate swelling and compressive modulus that
exceed the epidermis’ Young modulus, thus able to provide mechanical support upon
application. Meanwhile, the thermal analysis revealed that the onset decomposition
temperature of scaffold were at around 70 ºC which is due to the loss of water
present in the scaffold thus thermally safe for soft tissue application. Based on
FESEM result, there are different in surface structure of Chi/Alg based scaffold.
Finally, with the incorporation of 0.3 % PVP synthesised AgNPs in Chi/Alg based
scaffold, cells are able to live up to 14 days. As a result, Chi incorporation in the Alg
and AgNPs improved physical, mechanical properties of hydrogel itself and provide
biosafe environment during the study. |
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