Investigation of electroporation effect on HT29 cell lines adhesion properties
Electroporation is a method of permeabilizing a living cell membrane with high electric field intensity of short duration. This study investigates the effect of electroporation on colon cell line, HT29 adhesion Property as a preliminary study of the desire to design a potential application of electr...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Published: |
2015
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| Subjects: | |
| Online Access: | DOI:10.1109/ICoBE.2015.7235877 DOI:10.1109/ICoBE.2015.7235877 |
| Summary: | Electroporation is a method of permeabilizing a
living cell membrane with high electric field intensity of
short duration. This study investigates the effect of
electroporation on colon cell line, HT29 adhesion Property
as a preliminary study of the desire to design a potential
application of electroporation on wound healing. The
study found out that when HT29 cell lines were
electroporated with 600 V/cm and 500 μs pulse duration it
attached to the monolayer after 12 minutes of
Electroporation, and covers a distance of 38.7 μm after one
hour. While the non-electroporated cell line for control
under the same condition attached to monolayer after
37minutes from floating state and covers a distance of
31.53 μm after 55 minutes. The result showed that
electroporated HT29 cell line can attached faster and
spread wider than non-electroporated HT29 cell line that
can be used to facilitate cell migration for wound healing
application. |
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