In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application

Anodic oxidation has been widely used to modify the surface properties of titanium in order to improve the biocompatibility after implantation. In this study, high purity titanium foils were exposed in a mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate...

Full description

Bibliographic Details
Main Author:	Lee, Te Chuan
Format:	Thesis
Published:	2016
Subjects:	TS200-770 Metal manufactures. Metalworking
Online Access:	http://eprints.uthm.edu.my/9216/ http://eprints.uthm.edu.my/9216/1/Lee_Te_Chuan.pdf

id	uthm-9216
recordtype	eprints
spelling	uthm-92162017-08-02T09:08:21Z In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application Lee, Te Chuan TS200-770 Metal manufactures. Metalworking Anodic oxidation has been widely used to modify the surface properties of titanium in order to improve the biocompatibility after implantation. In this study, high purity titanium foils were exposed in a mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). The parameters for anodic oxidation method such as applied voltage (50-350 V), current density(10-70 mA.cm-2), electrolyte concentration (0.02 M β-GP + 0.2 M CA, 0.04 M β-GP + 0.04 M CA) , anodising time (5-10 mins), agitation speed (300-1500 rpm), ultrasonic amplitude (20-60 μm) and bath temperature (4-100 °C) were varied to investigate the impact on the surface properties of titanium. The results showed that surface of the titanium foil appeared to be highly porous and demonstrated high crystallinity as well as high hydrophilic properties especially when the parameters of anodic oxidation have been varied. This study also proposes two novel methods particularly to accelerate the bone-like apatite formation on the anodised titanium in a shorted time: (1) UV irradiation during in vitro testing and (2) adding additives in electrolyte. After soaked and irradiated with UV in simulated body fluid (SBF) for 7 days, highly crystallised bone-like apatite was fully covered on the anodised surface. Interestingly, the smooth and partially porous surface of the anodised titanium was observed to be fully covered by the bone-like apatite layer, which contradict previous research results. The mechanism for growth of bone-like apatite was developed and involved several stages from the existence of hydroxyl groups (•OH) under the UV irradiation has been disclosed thoroughly. Further, additives such as sulphuric acid (H2SO4), hydrogen peroxide (H2O2) and sodium hydroxide (NaOH) were added into the electrolyte were also able to accelerate the formation of bone-like apatite because of the presence of (•OH), tricalcium phosphate (Ca3O8P2), calcium diphosphate (Ca2O7P2), calcium titanate (CaTiO3) or sodium titanate (Na2Ti3O7) on the anodised surface, which able to induce the nucleation site of bone-like apatite. 2016 Thesis NonPeerReviewed application/pdf http://eprints.uthm.edu.my/9216/1/Lee_Te_Chuan.pdf Lee, Te Chuan (2016) In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application. PhD thesis, Universiti Tun Hussein Onn Malaysia. http://eprints.uthm.edu.my/9216/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Tun Hussein Onn Malaysia
building	UTHM Institutional Repository
collection	Online Access
topic	TS200-770 Metal manufactures. Metalworking
spellingShingle	TS200-770 Metal manufactures. Metalworking Lee, Te Chuan In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
description	Anodic oxidation has been widely used to modify the surface properties of titanium in order to improve the biocompatibility after implantation. In this study, high purity titanium foils were exposed in a mixture of β-glycerophosphate disodium salt pentahydrate (β-GP) and calcium acetate monohydrate (CA). The parameters for anodic oxidation method such as applied voltage (50-350 V), current density(10-70 mA.cm-2), electrolyte concentration (0.02 M β-GP + 0.2 M CA, 0.04 M β-GP + 0.04 M CA) , anodising time (5-10 mins), agitation speed (300-1500 rpm), ultrasonic amplitude (20-60 μm) and bath temperature (4-100 °C) were varied to investigate the impact on the surface properties of titanium. The results showed that surface of the titanium foil appeared to be highly porous and demonstrated high crystallinity as well as high hydrophilic properties especially when the parameters of anodic oxidation have been varied. This study also proposes two novel methods particularly to accelerate the bone-like apatite formation on the anodised titanium in a shorted time: (1) UV irradiation during in vitro testing and (2) adding additives in electrolyte. After soaked and irradiated with UV in simulated body fluid (SBF) for 7 days, highly crystallised bone-like apatite was fully covered on the anodised surface. Interestingly, the smooth and partially porous surface of the anodised titanium was observed to be fully covered by the bone-like apatite layer, which contradict previous research results. The mechanism for growth of bone-like apatite was developed and involved several stages from the existence of hydroxyl groups (•OH) under the UV irradiation has been disclosed thoroughly. Further, additives such as sulphuric acid (H2SO4), hydrogen peroxide (H2O2) and sodium hydroxide (NaOH) were added into the electrolyte were also able to accelerate the formation of bone-like apatite because of the presence of (•OH), tricalcium phosphate (Ca3O8P2), calcium diphosphate (Ca2O7P2), calcium titanate (CaTiO3) or sodium titanate (Na2Ti3O7) on the anodised surface, which able to induce the nucleation site of bone-like apatite.
format	Thesis
author	Lee, Te Chuan
author_facet	Lee, Te Chuan
author_sort	Lee, Te Chuan
title	In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
title_short	In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
title_full	In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
title_fullStr	In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
title_full_unstemmed	In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
title_sort	in vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application
publishDate	2016
url	http://eprints.uthm.edu.my/9216/ http://eprints.uthm.edu.my/9216/1/Lee_Te_Chuan.pdf
first_indexed	2018-09-05T11:49:24Z
last_indexed	2018-09-05T11:49:24Z
_version_	1610768097946370048

In vitro bioactivities of anodised titanium in mixture of β-glycerophosphate and calcium acetate for biomedical application

Similar Items