Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines

There are a growing number of deaths of cancer patients due to toxicity of conventional chemotherapy. Therefore, continuous discovery of natural anticancer compounds with cytoselective actions would be an ideal strategy to overcome the problem. Thus, natural products from plant are still the alterna...

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Main Authors:	Norzila, I., Shajarahtunnur, J., Hasmah, A.
Format:	Article
Published:	School of Health Sciences, Universiti Sains Malaysia 2016
Subjects:	RA440-440.87 Study and teaching. Research
Online Access:	http://eprints.usm.my/40711/ http://eprints.usm.my/40711/1/paper_published_HEJ.pdf

id	usm-40711
recordtype	eprints
spelling	usm-407112018-06-07T01:27:41Z Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines Norzila, I. Shajarahtunnur, J. Hasmah, A. RA440-440.87 Study and teaching. Research There are a growing number of deaths of cancer patients due to toxicity of conventional chemotherapy. Therefore, continuous discovery of natural anticancer compounds with cytoselective actions would be an ideal strategy to overcome the problem. Thus, natural products from plant are still the alternative source in the search for anticancer drugs that can have a direct effect on both cancer cell removal and also minimize the side effects to the patients. Based on the traditional usage and pharmaceutical potential of Brucea javanica reported before, a study on the leaves of this plant was carried out. The aims of the study were to isolate the bioactive compound from B. javanica Leaves (BJL) extracts via bioassay-guided fractionation using several selected cancer cell lines, to determine the mode of cancer cell death induced by BJL’s active compound and the molecular mechanism of apoptosis implicated in cancer cell lines by measuring the level of apoptotic protein expression such as bax, bcl-XL, caspase-3 and tumour suppressor p53. Among three crude extracts of BJL, methanol was the most potent against selected cancer cell lines which consist of cervical, breast, bone, ovarian and liver cancer cells. Cisplatin was used as positive control for the antiproliferative assay. Using a bioassay-guided fractionation, chromatography, NMR and mass spectrometry analysis we have isolated luteolin. It is a known compound from the flavonoid group which was found to be cytoselective School of Health Sciences, Universiti Sains Malaysia 2016 Article PeerReviewed application/pdf http://eprints.usm.my/40711/1/paper_published_HEJ.pdf Norzila, I. and Shajarahtunnur, J. and Hasmah, A. (2016) Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines. Health and the Environment Journal, 7 (2). pp. 116-136. ISSN 2180-1126 http://eprints.usm.my/40711/
institution	Universiti Sains Malaysia Universiti Sains Malaysia
repository_type	Digital Repository
institution_category	Local University
building	USM Repository
collection	Online Access
topic	RA440-440.87 Study and teaching. Research
spellingShingle	RA440-440.87 Study and teaching. Research Norzila, I. Shajarahtunnur, J. Hasmah, A. Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines
description	There are a growing number of deaths of cancer patients due to toxicity of conventional chemotherapy. Therefore, continuous discovery of natural anticancer compounds with cytoselective actions would be an ideal strategy to overcome the problem. Thus, natural products from plant are still the alternative source in the search for anticancer drugs that can have a direct effect on both cancer cell removal and also minimize the side effects to the patients. Based on the traditional usage and pharmaceutical potential of Brucea javanica reported before, a study on the leaves of this plant was carried out. The aims of the study were to isolate the bioactive compound from B. javanica Leaves (BJL) extracts via bioassay-guided fractionation using several selected cancer cell lines, to determine the mode of cancer cell death induced by BJL’s active compound and the molecular mechanism of apoptosis implicated in cancer cell lines by measuring the level of apoptotic protein expression such as bax, bcl-XL, caspase-3 and tumour suppressor p53. Among three crude extracts of BJL, methanol was the most potent against selected cancer cell lines which consist of cervical, breast, bone, ovarian and liver cancer cells. Cisplatin was used as positive control for the antiproliferative assay. Using a bioassay-guided fractionation, chromatography, NMR and mass spectrometry analysis we have isolated luteolin. It is a known compound from the flavonoid group which was found to be cytoselective
format	Article
author	Norzila, I. Shajarahtunnur, J. Hasmah, A.
author_facet	Norzila, I. Shajarahtunnur, J. Hasmah, A.
author_sort	Norzila, I.
title	Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines
title_short	Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines
title_full	Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines
title_fullStr	Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines
title_full_unstemmed	Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines
title_sort	natural luteolin from methanolic extract of malaysian brucea javanica leaves induces apoptosis in hela cell lines
publisher	School of Health Sciences, Universiti Sains Malaysia
publishDate	2016
url	http://eprints.usm.my/40711/ http://eprints.usm.my/40711/1/paper_published_HEJ.pdf
first_indexed	2018-09-08T09:49:30Z
last_indexed	2018-09-08T09:49:30Z
_version_	1611032344988221440

Natural Luteolin from Methanolic Extract of Malaysian Brucea javanica Leaves Induces Apoptosis in HeLa Cell Lines

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