RNomic identification and evaluation of npcTB_6715, a nonprotein- coding RNA gene as a potential biomarker for the detection of Mycobacterium tuberculosis
Technological advances in RNA biology greatly improved transcriptome profiling during the last two decades. Besides the discovery of many small RNAs (sRNA) that are involved in the physiological and pathophysiological regulation of various cellular circuits, it becomes evident that the correspondi...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Foundation for Cellular and Molecular Medicine
2017
|
| Subjects: | |
| Online Access: | http://onlinelibrary.wiley.com/wol1/doi/10.1111/jcmm.13148/full http://onlinelibrary.wiley.com/wol1/doi/10.1111/jcmm.13148/full http://eprints.usm.my/37308/1/Kanniappan_et_al%2D2017%2DJournal_of_Cellular_and_Molecular_Medicine.pdf |
| id |
usm-37308 |
|---|---|
| recordtype |
eprints |
| spelling |
usm-373082017-11-01T09:38:00Z RNomic identification and evaluation of npcTB_6715, a nonprotein- coding RNA gene as a potential biomarker for the detection of Mycobacterium tuberculosis Kanniappan, Priyatharisni Ahmed, Siti Aminah Rajasekaram, Ganeswrie Marimuthu, Citartan Ewe, Seng Ch’ng Li, Pin Lee Raabe, Carsten A. Rozhdestvensky, Timofey S. Thean, Hock Tang RA440-440.87 Study and teaching. Research Technological advances in RNA biology greatly improved transcriptome profiling during the last two decades. Besides the discovery of many small RNAs (sRNA) that are involved in the physiological and pathophysiological regulation of various cellular circuits, it becomes evident that the corresponding RNA genes might also serve as potential biomarkers to monitor the progression of disease and treatment. sRNA gene candidate npcTB_6715 was previously identified via experimental RNomic (unpublished data), and we report its application as potential biomarker for the detection of Mycobacterium tuberculosis (MTB) in patient samples. For proof of principle, we developed a multiplex PCR assay and report its validation with 500 clinical cultures, positive for Mycobacteria. The analysis revealed 98.9% sensitivity, 96.1% specificity, positive and negative predictive values of 98.6% and 96.8%, respectively. These results underscore the diagnostic value of the sRNA gene as diagnostic marker for the specific detection of MTB in clinical samples. Its successful application and the general ease of PCR-based detection compared to standard bacterial culture techniques might be the first step towards ‘point-of-care’ diagnostics of Mycobacteria. To the best of our knowledge, this is the first time for the design of diagnostic applications based on sRNA genes, in Mycobacteria. Foundation for Cellular and Molecular Medicine 2017 Article PeerReviewed application/pdf http://eprints.usm.my/37308/1/Kanniappan_et_al%2D2017%2DJournal_of_Cellular_and_Molecular_Medicine.pdf http://onlinelibrary.wiley.com/wol1/doi/10.1111/jcmm.13148/full Kanniappan, Priyatharisni and Ahmed, Siti Aminah and Rajasekaram, Ganeswrie and Marimuthu, Citartan and Ewe, Seng Ch’ng and Li, Pin Lee and Raabe, Carsten A. and Rozhdestvensky, Timofey S. and Thean, Hock Tang (2017) RNomic identification and evaluation of npcTB_6715, a nonprotein- coding RNA gene as a potential biomarker for the detection of Mycobacterium tuberculosis. Journal of Cellular and Molecular Medicine, 21 (10). pp. 2276-2283. ISSN 1582-4934 http://eprints.usm.my/37308/ |
| institution |
Universiti Sains Malaysia Universiti Sains Malaysia |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| building |
USM Repository |
| collection |
Online Access |
| topic |
RA440-440.87 Study and teaching. Research |
| spellingShingle |
RA440-440.87 Study and teaching. Research Kanniappan, Priyatharisni Ahmed, Siti Aminah Rajasekaram, Ganeswrie Marimuthu, Citartan Ewe, Seng Ch’ng Li, Pin Lee Raabe, Carsten A. Rozhdestvensky, Timofey S. Thean, Hock Tang RNomic identification and evaluation of npcTB_6715, a nonprotein- coding RNA gene as a potential biomarker for the detection of Mycobacterium tuberculosis |
| description |
Technological advances in RNA biology greatly improved transcriptome profiling during the last two decades. Besides the discovery of many
small RNAs (sRNA) that are involved in the physiological and pathophysiological regulation of various cellular circuits, it becomes evident that
the corresponding RNA genes might also serve as potential biomarkers to monitor the progression of disease and treatment. sRNA gene candidate
npcTB_6715 was previously identified via experimental RNomic (unpublished data), and we report its application as potential biomarker
for the detection of Mycobacterium tuberculosis (MTB) in patient samples. For proof of principle, we developed a multiplex PCR assay and
report its validation with 500 clinical cultures, positive for Mycobacteria. The analysis revealed 98.9% sensitivity, 96.1% specificity, positive and
negative predictive values of 98.6% and 96.8%, respectively. These results underscore the diagnostic value of the sRNA gene as diagnostic
marker for the specific detection of MTB in clinical samples. Its successful application and the general ease of PCR-based detection compared
to standard bacterial culture techniques might be the first step towards ‘point-of-care’ diagnostics of Mycobacteria. To the best of our knowledge,
this is the first time for the design of diagnostic applications based on sRNA genes, in Mycobacteria. |
| format |
Article |
| author |
Kanniappan, Priyatharisni Ahmed, Siti Aminah Rajasekaram, Ganeswrie Marimuthu, Citartan Ewe, Seng Ch’ng Li, Pin Lee Raabe, Carsten A. Rozhdestvensky, Timofey S. Thean, Hock Tang |
| author_facet |
Kanniappan, Priyatharisni Ahmed, Siti Aminah Rajasekaram, Ganeswrie Marimuthu, Citartan Ewe, Seng Ch’ng Li, Pin Lee Raabe, Carsten A. Rozhdestvensky, Timofey S. Thean, Hock Tang |
| author_sort |
Kanniappan, Priyatharisni |
| title |
RNomic identification and evaluation of npcTB_6715, a nonprotein-
coding RNA gene as a potential biomarker for the
detection of Mycobacterium tuberculosis |
| title_short |
RNomic identification and evaluation of npcTB_6715, a nonprotein-
coding RNA gene as a potential biomarker for the
detection of Mycobacterium tuberculosis |
| title_full |
RNomic identification and evaluation of npcTB_6715, a nonprotein-
coding RNA gene as a potential biomarker for the
detection of Mycobacterium tuberculosis |
| title_fullStr |
RNomic identification and evaluation of npcTB_6715, a nonprotein-
coding RNA gene as a potential biomarker for the
detection of Mycobacterium tuberculosis |
| title_full_unstemmed |
RNomic identification and evaluation of npcTB_6715, a nonprotein-
coding RNA gene as a potential biomarker for the
detection of Mycobacterium tuberculosis |
| title_sort |
rnomic identification and evaluation of npctb_6715, a nonprotein-
coding rna gene as a potential biomarker for the
detection of mycobacterium tuberculosis |
| publisher |
Foundation for Cellular and Molecular Medicine |
| publishDate |
2017 |
| url |
http://onlinelibrary.wiley.com/wol1/doi/10.1111/jcmm.13148/full http://onlinelibrary.wiley.com/wol1/doi/10.1111/jcmm.13148/full http://eprints.usm.my/37308/1/Kanniappan_et_al%2D2017%2DJournal_of_Cellular_and_Molecular_Medicine.pdf |
| first_indexed |
2018-09-08T09:18:23Z |
| last_indexed |
2018-09-08T09:18:23Z |
| _version_ |
1611030387632373760 |