A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene
An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7246/ http://psasir.upm.edu.my/id/eprint/7246/ http://psasir.upm.edu.my/id/eprint/7246/1/A%20novel%20organic%20solvent%20tolerant%20lipase%20from%20Bacillus%20sphaericus%20205y%20extracellular%20expression%20of%20a%20novel%20OST-lipase%20gene.pdf |