Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12
In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybd...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hibiscus Publisher
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/64006/ http://psasir.upm.edu.my/id/eprint/64006/ http://psasir.upm.edu.my/id/eprint/64006/1/Partial%20purification%20and%20characterization%20of%20the%20molybdenum-reducing%20enzyme%20from%20the%20glyphosate-degrading%20Burkholderia%20vietnamiensis%20strain%20AQ5-12.pdf |
| Summary: | In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only exhibits a single biotransformation activity. The enzyme was partially purified using ammonium sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative assessment with the previously characterized Mo-reducing enzyme from various bacteria showed that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower enzyme activity. |
|---|