Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia
Very few studies on Campylobacter in chickens were done in Malaysia. This study was carried out to determine the prevalence of Campylobacter in broiler chickens and to characterize the isolates to species level, their antibiotic resistance patterns and plasmid profiles. Ten broiler chicken farms wer...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Asian Network for Scientific Information
2002
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/40381/ http://psasir.upm.edu.my/id/eprint/40381/ http://psasir.upm.edu.my/id/eprint/40381/ http://psasir.upm.edu.my/id/eprint/40381/1/Isolation%20and%20characterization%20of%20Campylobacter%20jejuni%20from%20broiler%20chickens%20in%20Malaysia.pdf |
| id |
upm-40381 |
|---|---|
| recordtype |
eprints |
| spelling |
upm-403812015-09-17T23:56:07Z http://psasir.upm.edu.my/id/eprint/40381/ Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia Abdul Aziz, Saleha Very few studies on Campylobacter in chickens were done in Malaysia. This study was carried out to determine the prevalence of Campylobacter in broiler chickens and to characterize the isolates to species level, their antibiotic resistance patterns and plasmid profiles. Ten broiler chicken farms were studied. The chickens in nine of the farms were found colonized with Campylobacters; the prevalence rates ranged from 46.3 to 93.3%, with a mean of 72.6%; one farm was negative. Campylobacter jejuni was the predominant species. Antibiotic susceptibility test revealed that all the 76 C. jejuni isolates tested were resistant to tetracycline; 82.9, 55.3 and 34.2% were resistant to streptomycin, kanamycin and ampicillin, respectively while resistance to gentamicin, erythromycin and chloramphenicol ranged from 26.3 to 22.4%. It was also found that 22.4% of the isolates were resistant to one antibiotic while 13.1% to all seven antibiotics. 59% of C. jejuni isolates harboured between one to four plasmids, demonstrating 14 plasmid profiles with size of the plasmids ranged from 4.5 to 70.3 kb. More isolates containing plasmids were observed to be resistant to tetracycline, chloramphenicol and kanamycin than those isolates without plasmids. The numbers of plasmid bands present were not consistent with the antibiotic resistance patterns. Asian Network for Scientific Information 2002 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40381/1/Isolation%20and%20characterization%20of%20Campylobacter%20jejuni%20from%20broiler%20chickens%20in%20Malaysia.pdf Abdul Aziz, Saleha (2002) Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia. International Journal of Poultry Science, 1 (4). pp. 94-97. ISSN 1682-8356; ESSN: 1994-7992 http://scialert.net/abstract/?doi=ijps.2002.94.97 10.3923/ijps.2002.94.97 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Putra Malaysia |
| building |
UPM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
Very few studies on Campylobacter in chickens were done in Malaysia. This study was carried out to determine the prevalence of Campylobacter in broiler chickens and to characterize the isolates to species level, their antibiotic resistance patterns and plasmid profiles. Ten broiler chicken farms were studied. The chickens in nine of the farms were found colonized with Campylobacters; the prevalence rates ranged from 46.3 to 93.3%, with a mean of 72.6%; one farm was negative. Campylobacter jejuni was the predominant species. Antibiotic susceptibility test revealed that all the 76 C. jejuni isolates tested were resistant to tetracycline; 82.9, 55.3 and 34.2% were resistant to streptomycin, kanamycin and ampicillin, respectively while resistance to gentamicin, erythromycin and chloramphenicol ranged from 26.3 to 22.4%. It was also found that 22.4% of the isolates were resistant to one antibiotic while 13.1% to all seven antibiotics. 59% of C. jejuni isolates harboured between one to four plasmids, demonstrating 14 plasmid profiles with size of the plasmids ranged from 4.5 to 70.3 kb. More isolates containing plasmids were observed to be resistant to tetracycline, chloramphenicol and kanamycin than those isolates without plasmids. The numbers of plasmid bands present were not consistent with the antibiotic resistance patterns. |
| format |
Article |
| author |
Abdul Aziz, Saleha |
| spellingShingle |
Abdul Aziz, Saleha Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia |
| author_facet |
Abdul Aziz, Saleha |
| author_sort |
Abdul Aziz, Saleha |
| title |
Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia |
| title_short |
Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia |
| title_full |
Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia |
| title_fullStr |
Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia |
| title_full_unstemmed |
Isolation and characterization of Campylobacter jejuni from broiler chickens in Malaysia |
| title_sort |
isolation and characterization of campylobacter jejuni from broiler chickens in malaysia |
| publisher |
Asian Network for Scientific Information |
| publishDate |
2002 |
| url |
http://psasir.upm.edu.my/id/eprint/40381/ http://psasir.upm.edu.my/id/eprint/40381/ http://psasir.upm.edu.my/id/eprint/40381/ http://psasir.upm.edu.my/id/eprint/40381/1/Isolation%20and%20characterization%20of%20Campylobacter%20jejuni%20from%20broiler%20chickens%20in%20Malaysia.pdf |
| first_indexed |
2018-09-07T17:00:06Z |
| last_indexed |
2018-09-07T17:00:06Z |
| _version_ |
1610968839256801280 |