Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide

Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide

Oxidative stress and inflammation triggers the production of free radicals and toxic pro-inflammatory cytokines leading to neurodegeneration. Down-regulation of oxidative stress and inflammatory mediators offers defence against development and progression of the disease. Andrographolide is the most...

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Main Authors: Sani, Dahiru, Ramli, Noorlela, Kirby, Brian, Sumon, Md. Shariful Hasan, Basri, Hamidon, Stanslas, Johnson
Format: Article
Language:English
Published: Federation of American Societies for Experimental Biology 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36460/
http://psasir.upm.edu.my/id/eprint/36460/
http://psasir.upm.edu.my/id/eprint/36460/1/Andrographolide%20downregulates%20pro.pdf
id upm-36460
recordtype eprints
spelling upm-364602016-01-26T07:33:35Z http://psasir.upm.edu.my/id/eprint/36460/ Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide Sani, Dahiru Ramli, Noorlela Kirby, Brian Sumon, Md. Shariful Hasan Basri, Hamidon Stanslas, Johnson Oxidative stress and inflammation triggers the production of free radicals and toxic pro-inflammatory cytokines leading to neurodegeneration. Down-regulation of oxidative stress and inflammatory mediators offers defence against development and progression of the disease. Andrographolide is the most abundant diterpene lactone isolated from the leaves of Andrographis paniculata. Despite the increasing literature studies on the anti-inflammatory effect of andrographolide, there is still paucity regarding its neuroprotective role as can be ascertained from the search of literature. The present study investigated the potential therapeutic role of andrographolide as a neuroprotective agent via in vitro model of lipopolysaccharide (LPS)-induced brain injury using microglial (BV2) and dopaminergic (N27) cells. Pre-treatment of andrographolide at non-cytotoxic concentration range (0.25-2.0 µM) of BV2 cells followed by LPS dose-dependently suppressed nitric oxide production, pro-inflammatory cytokines (TNF-α, IL-6) as well as attenuating intracellular reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS) compared to cells without andrographolide pretreatment. Further, conditioned media (CM) of the pretreatment had diminished toxicity towards dopaminergic neurons as compared to CM without pretreatment. Keywords: Andrographolide; Proinflammatory cytokines; oxidative stress; microglial; dopaminergic neurodegeneration. Federation of American Societies for Experimental Biology 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36460/1/Andrographolide%20downregulates%20pro.pdf Sani, Dahiru and Ramli, Noorlela and Kirby, Brian and Sumon, Md. Shariful Hasan and Basri, Hamidon and Stanslas, Johnson (2014) Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide. The FASEB Journal, 28 (1). ISSN 0892-6638; ESSN: 1530-6860 http://www.fasebj.org/content/28/1_Supplement/1143.16
repository_type Digital Repository
institution_category Local University
institution Universiti Putra Malaysia
building UPM Institutional Repository
collection Online Access
language English
description Oxidative stress and inflammation triggers the production of free radicals and toxic pro-inflammatory cytokines leading to neurodegeneration. Down-regulation of oxidative stress and inflammatory mediators offers defence against development and progression of the disease. Andrographolide is the most abundant diterpene lactone isolated from the leaves of Andrographis paniculata. Despite the increasing literature studies on the anti-inflammatory effect of andrographolide, there is still paucity regarding its neuroprotective role as can be ascertained from the search of literature. The present study investigated the potential therapeutic role of andrographolide as a neuroprotective agent via in vitro model of lipopolysaccharide (LPS)-induced brain injury using microglial (BV2) and dopaminergic (N27) cells. Pre-treatment of andrographolide at non-cytotoxic concentration range (0.25-2.0 µM) of BV2 cells followed by LPS dose-dependently suppressed nitric oxide production, pro-inflammatory cytokines (TNF-α, IL-6) as well as attenuating intracellular reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS) compared to cells without andrographolide pretreatment. Further, conditioned media (CM) of the pretreatment had diminished toxicity towards dopaminergic neurons as compared to CM without pretreatment. Keywords: Andrographolide; Proinflammatory cytokines; oxidative stress; microglial; dopaminergic neurodegeneration.
format Article
author Sani, Dahiru
Ramli, Noorlela
Kirby, Brian
Sumon, Md. Shariful Hasan
Basri, Hamidon
Stanslas, Johnson
spellingShingle Sani, Dahiru
Ramli, Noorlela
Kirby, Brian
Sumon, Md. Shariful Hasan
Basri, Hamidon
Stanslas, Johnson
Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
author_facet Sani, Dahiru
Ramli, Noorlela
Kirby, Brian
Sumon, Md. Shariful Hasan
Basri, Hamidon
Stanslas, Johnson
author_sort Sani, Dahiru
title Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
title_short Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
title_full Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
title_fullStr Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
title_full_unstemmed Andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
title_sort andrographolide downregulates pro-inflammatory cytokines and free radical productions to prevent dopaminergic neuro-degeneration induced by lipopolysaccharide
publisher Federation of American Societies for Experimental Biology
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36460/
http://psasir.upm.edu.my/id/eprint/36460/
http://psasir.upm.edu.my/id/eprint/36460/1/Andrographolide%20downregulates%20pro.pdf
first_indexed 2018-09-07T16:31:08Z
last_indexed 2018-09-07T16:31:08Z
_version_ 1610967016932376576

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