A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method
A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions to bind and elute tNiVG protein were 50 mM sodium carbonate with pH...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/36282/ http://psasir.upm.edu.my/id/eprint/36282/ http://psasir.upm.edu.my/id/eprint/36282/1/A%20single.pdf |