Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis

A total of 21 DNA microsatellites were isolated from Perna viridis lJy using a 5' anchored polymerase chain reaction technique. Primers were designed for seven microsatellite loci and the peR amplifications of these seven microsatellite loci showed that five were polymorphic with the number of...

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Main Authors:	Ong, Lily Chin Chin, Tan, Soon Guan, Yusoff, Khatijah, Yap, Chee Kong
Format:	Article
Language:	English
Published:	Universiti Putra Malaysia Press 2005
Online Access:	http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/1/Isolation_of_DNA_Microsatellite_Markers_in_the.pdf

id	upm-3602
recordtype	eprints
spelling	upm-36022015-09-15T01:47:31Z http://psasir.upm.edu.my/id/eprint/3602/ Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong A total of 21 DNA microsatellites were isolated from Perna viridis lJy using a 5' anchored polymerase chain reaction technique. Primers were designed for seven microsatellite loci and the peR amplifications of these seven microsatellite loci showed that five were polymorphic with the number ofalleles per locus rangingfrom 2 to 4. These are the first set of microsatellite markers developed for this species. These markers are useful as tools for more detailed studies of the genetic backgrounds of the green-lipped musse~ P. viridis, in our country as this species is being cultured as a cheap source of protein for human consumption and has been identified as a potential biomonitoring agent for heavy metal pollution in the west coast of Peninsular Malaysia. Universiti Putra Malaysia Press 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3602/1/Isolation_of_DNA_Microsatellite_Markers_in_the.pdf Ong, Lily Chin Chin and Tan, Soon Guan and Yusoff, Khatijah and Yap, Chee Kong (2005) Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 79-85. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(79-85).pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English
description	A total of 21 DNA microsatellites were isolated from Perna viridis lJy using a 5' anchored polymerase chain reaction technique. Primers were designed for seven microsatellite loci and the peR amplifications of these seven microsatellite loci showed that five were polymorphic with the number ofalleles per locus rangingfrom 2 to 4. These are the first set of microsatellite markers developed for this species. These markers are useful as tools for more detailed studies of the genetic backgrounds of the green-lipped musse~ P. viridis, in our country as this species is being cultured as a cheap source of protein for human consumption and has been identified as a potential biomonitoring agent for heavy metal pollution in the west coast of Peninsular Malaysia.
format	Article
author	Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong
spellingShingle	Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
author_facet	Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong
author_sort	Ong, Lily Chin Chin
title	Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
title_short	Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
title_full	Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
title_fullStr	Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
title_full_unstemmed	Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
title_sort	isolation of dna microsatellite markers in the green-lipped mussel, perna viridis
publisher	Universiti Putra Malaysia Press
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/1/Isolation_of_DNA_Microsatellite_Markers_in_the.pdf
first_indexed	2018-09-07T12:03:41Z
last_indexed	2018-09-07T12:03:41Z
_version_	1610950190315864064

Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis

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