Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops

This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) th...

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Main Authors:	Munusamy, Umaiyal, Abdullah, Siti Nor Akmar, Abdul Aziz, Maheran, Khaza'ai, Huzwah, Lai, Mun Seong
Format:	Article
Language:	English
Published:	Science Publications 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/1/ajbbsp.2013.355.364.pdf

id	upm-29769
recordtype	eprints
spelling	upm-297692017-12-04T04:37:32Z http://psasir.upm.edu.my/id/eprint/29769/ Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) that shared greater than 75% sequence identity with TC sequences from other plant species was used to silence TC gene expression in Amaranthus sp. and Allium porrum leaves by co-suppression strategies. This was carried out using recombinant vector constructs designated as p4a11 and p4c9 containing TC driven by the maize Ubiquitin promoter (Ubi1P) and the oil palm leaf-specific promoter (LHCB), respectively. In the Amaranthus sp. leaves infiltrated with p4a11, the α-tocopherol suppression was 85.0, 71.0 and 74.0% on day three, five and seven, respectively and complete suppression was observed in the leaves infiltrated with p4c9. Whereas, only 11.0, 7.0 and 29.0% suppression was detected in Allium porrum leaves infiltrated with p4a11 and 45.0, 4.0 and 57.0% of suppression was observed in Allium porrum infiltrated with p4c9 on day three, five and seven, respectively. Silencing of TC gene driven by LHCB (p4c9) causes the reduction of α-tocopherol production better than by Ubi1P (p4a11). The results have demonstrated that gene silencing through co-suppression is capable of producing α-tocotrienol. However, there is no correlation between the α-tocotrienol production and the α-tocopherol suppression. It has been suggested that the transgene mediated co-suppression mechanism had triggered the vitamin E biosynthesis metabolic pathway to shift towards producing α-tocotrienol. Science Publications 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29769/1/ajbbsp.2013.355.364.pdf Munusamy, Umaiyal and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Khaza'ai, Huzwah and Lai, Mun Seong (2013) Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops. American Journal of Biochemistry and Biotechnology, 9 (4). pp. 355-364. ISSN 1553-3468; ESSN: 1558-6332 http://thescipub.com/abstract/10.3844/ajbbsp.2013.355.364 10.3844/ajbbsp.2013.355.364
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English
description	This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) that shared greater than 75% sequence identity with TC sequences from other plant species was used to silence TC gene expression in Amaranthus sp. and Allium porrum leaves by co-suppression strategies. This was carried out using recombinant vector constructs designated as p4a11 and p4c9 containing TC driven by the maize Ubiquitin promoter (Ubi1P) and the oil palm leaf-specific promoter (LHCB), respectively. In the Amaranthus sp. leaves infiltrated with p4a11, the α-tocopherol suppression was 85.0, 71.0 and 74.0% on day three, five and seven, respectively and complete suppression was observed in the leaves infiltrated with p4c9. Whereas, only 11.0, 7.0 and 29.0% suppression was detected in Allium porrum leaves infiltrated with p4a11 and 45.0, 4.0 and 57.0% of suppression was observed in Allium porrum infiltrated with p4c9 on day three, five and seven, respectively. Silencing of TC gene driven by LHCB (p4c9) causes the reduction of α-tocopherol production better than by Ubi1P (p4a11). The results have demonstrated that gene silencing through co-suppression is capable of producing α-tocotrienol. However, there is no correlation between the α-tocotrienol production and the α-tocopherol suppression. It has been suggested that the transgene mediated co-suppression mechanism had triggered the vitamin E biosynthesis metabolic pathway to shift towards producing α-tocotrienol.
format	Article
author	Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong
spellingShingle	Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
author_facet	Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong
author_sort	Munusamy, Umaiyal
title	Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
title_short	Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
title_full	Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
title_fullStr	Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
title_full_unstemmed	Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
title_sort	induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
publisher	Science Publications
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/1/ajbbsp.2013.355.364.pdf
first_indexed	2018-09-07T15:33:54Z
last_indexed	2018-09-07T15:33:54Z
_version_	1610963416470519808

Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops

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