Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia
The in vitro activities of 6 antimicrobial agents against clinical isolates of Streptococcus pneumoniae (pneumococci) were investigated and the erythromycin minimum inhibitory concentrations (MICs) were correlated with the two major macrolide resistance determinants, mef(A) and erm(B). MICs of commo...
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| Language: | English |
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Malaysian Medical Association
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29765/ http://psasir.upm.edu.my/id/eprint/29765/ http://psasir.upm.edu.my/id/eprint/29765/1/Prevalence%20of%20macrolide%20resistance%20and%20in%20vitro%20activities%20of%20six%20antimicrobial%20agents%20against%20clinical%20isolates%20of%20streptococcus%20pneumoniae%20from%20a%20multi.pdf |
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upm-297652017-08-09T08:15:28Z http://psasir.upm.edu.my/id/eprint/29765/ Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia Jeevajothi Nathan, Jayakayatri Mohd Taib, Niazlin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Md Yasin, Rohani Jamal, Farida Fatema @ Farida Sagineedu, Sreenivasa Rao Karunanidhi, Arunkumar The in vitro activities of 6 antimicrobial agents against clinical isolates of Streptococcus pneumoniae (pneumococci) were investigated and the erythromycin minimum inhibitory concentrations (MICs) were correlated with the two major macrolide resistance determinants, mef(A) and erm(B). MICs of commonly used antibiotics as well as the presence of macrolide resistance determinant genes in all isolates were tested. Seventy one pneumococcal isolates collected at Institute for Medical Research (IMR) were included in this study. Phenotypic characterization, MIC determination using E-test strips and polymerase chain reactions for antibiotic resistance determination were included. Among the isolates, 25 (35.2%) isolates were erythromycin susceptible, 3 (4.2%) were intermediate and 42 (60.6%) were resistant. Fifty three isolates (74.7%) were found with mef(A) alone, 15 (21.1%) isolates with erm(B) + mef(A) combination and 3 (4.2%) isolates with none of the two genes. The in vitro activity of penicillin, amoxicillin clavulanic acid, ceftriaxone and cefotaxime is superior to trimethoprim-sulfamethoxazole and erythromycin. In conclusion, pneumococcal isolates in this study were highly susceptible to penicillin with very low MICs. However, a very high prevalence rate of erythromycin resistance was observed. Erythromycin resistant S. pneumoniae isolates with both mef(A) and erm(B) showed very high MICs ≥256 μg/mL. Malaysian Medical Association 2013-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29765/1/Prevalence%20of%20macrolide%20resistance%20and%20in%20vitro%20activities%20of%20six%20antimicrobial%20agents%20against%20clinical%20isolates%20of%20streptococcus%20pneumoniae%20from%20a%20multi.pdf Jeevajothi Nathan, Jayakayatri and Mohd Taib, Niazlin and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Md Yasin, Rohani and Jamal, Farida Fatema @ Farida and Sagineedu, Sreenivasa Rao and Karunanidhi, Arunkumar (2013) Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia. Medical Journal of Malaysia, 68 (2). pp. 119-124. ISSN 0300-5283 http://www.e-mjm.org/2013/v68n2/index.html |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Putra Malaysia |
| building |
UPM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
The in vitro activities of 6 antimicrobial agents against clinical isolates of Streptococcus pneumoniae (pneumococci) were investigated and the erythromycin minimum inhibitory concentrations (MICs) were correlated with the two major macrolide resistance determinants, mef(A) and erm(B). MICs of commonly used antibiotics as well as the presence of macrolide resistance determinant genes in all isolates were tested. Seventy one pneumococcal isolates collected at Institute for Medical Research (IMR) were included in this study. Phenotypic characterization, MIC determination using E-test strips and polymerase chain reactions for antibiotic resistance determination were included. Among the isolates, 25 (35.2%) isolates were erythromycin susceptible, 3 (4.2%) were intermediate and 42 (60.6%) were resistant. Fifty three isolates (74.7%) were found with mef(A) alone, 15 (21.1%) isolates with erm(B) + mef(A) combination and 3 (4.2%) isolates with none of the two genes. The in vitro activity of penicillin, amoxicillin clavulanic acid, ceftriaxone and cefotaxime is superior to trimethoprim-sulfamethoxazole and erythromycin. In conclusion, pneumococcal isolates in this study were highly susceptible to penicillin with very low MICs. However, a very high prevalence rate of erythromycin resistance was observed. Erythromycin resistant S. pneumoniae isolates with both mef(A) and erm(B) showed very high MICs ≥256 μg/mL. |
| format |
Article |
| author |
Jeevajothi Nathan, Jayakayatri Mohd Taib, Niazlin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Md Yasin, Rohani Jamal, Farida Fatema @ Farida Sagineedu, Sreenivasa Rao Karunanidhi, Arunkumar |
| spellingShingle |
Jeevajothi Nathan, Jayakayatri Mohd Taib, Niazlin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Md Yasin, Rohani Jamal, Farida Fatema @ Farida Sagineedu, Sreenivasa Rao Karunanidhi, Arunkumar Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia |
| author_facet |
Jeevajothi Nathan, Jayakayatri Mohd Taib, Niazlin Mohd Desa, Mohd Nasir Masri, Siti Norbaya Md Yasin, Rohani Jamal, Farida Fatema @ Farida Sagineedu, Sreenivasa Rao Karunanidhi, Arunkumar |
| author_sort |
Jeevajothi Nathan, Jayakayatri |
| title |
Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia |
| title_short |
Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia |
| title_full |
Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia |
| title_fullStr |
Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia |
| title_full_unstemmed |
Prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in Malaysia |
| title_sort |
prevalence of macrolide resistance and in vitro activities of six antimicrobial agents against clinical isolates of streptococcus pneumoniae from a multi-center surveillance in malaysia |
| publisher |
Malaysian Medical Association |
| publishDate |
2013 |
| url |
http://psasir.upm.edu.my/id/eprint/29765/ http://psasir.upm.edu.my/id/eprint/29765/ http://psasir.upm.edu.my/id/eprint/29765/1/Prevalence%20of%20macrolide%20resistance%20and%20in%20vitro%20activities%20of%20six%20antimicrobial%20agents%20against%20clinical%20isolates%20of%20streptococcus%20pneumoniae%20from%20a%20multi.pdf |
| first_indexed |
2018-09-07T15:33:52Z |
| last_indexed |
2018-09-07T15:33:52Z |
| _version_ |
1610963414258024448 |