Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda

Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the...

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Main Authors:	Thanh, Tran, Chi, Vu Thi Quynh, Abdullah, Mohd Puad, Omar, Hishamuddin, Noroozi, Mostafa, Ky, Huynh, Napis, Suhaimi
Format:	Article
Language:	English
Published:	Springer 2011
Online Access:	http://psasir.upm.edu.my/id/eprint/25181/ http://psasir.upm.edu.my/id/eprint/25181/ http://psasir.upm.edu.my/id/eprint/25181/ http://psasir.upm.edu.my/id/eprint/25181/1/Construction%20of%20cDNA%20library%20and%20preliminary%20analysis%20of%20expressed%20sequence%20tags%20from%20green%20microalga%20Ankistrodesmus%20convolutus%20Corda.pdf

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spelling	upm-251812017-09-28T08:06:48Z http://psasir.upm.edu.my/id/eprint/25181/ Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the primary and amplified cDNA libraries were 1.1×10(6) and 6.0×10(9) pfu/ml respectively. The percentage of recombinants was 97% in the primary library and a total of 337 out of 415 original cDNA clones selected randomly contained inserts ranging from 600 to 1,500 bps. A total of 201 individual ESTs with sizes ranging from 390 to 1,038 bps were then analyzed and the BLASTX score revealed that 35.8% of the sequences were classified as strong match, 38.3% as nominal and 25.9% as weak match. Among the ESTs with known putative function, 21.4% of them were found to be related to gene expression, 14.4% ESTs to photosynthesis, 10.9% ESTs to metabolism, 5.5% ESTs to miscellaneous, 2.0% to stress response, and the remaining 45.8% were classified as novel genes. Analysis of ESTs described in this paper can be an effective approach to isolate and characterize new genes from A. convolutus and thus the sequences obtained represented a significant contribution to the extensive database of sequences from green microalgae. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25181/1/Construction%20of%20cDNA%20library%20and%20preliminary%20analysis%20of%20expressed%20sequence%20tags%20from%20green%20microalga%20Ankistrodesmus%20convolutus%20Corda.pdf Thanh, Tran and Chi, Vu Thi Quynh and Abdullah, Mohd Puad and Omar, Hishamuddin and Noroozi, Mostafa and Ky, Huynh and Napis, Suhaimi (2011) Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda. Molecular Biology Reports, 38 (1). pp. 177-182. ISSN 0301-4851; ESSN: 1573-4978 https://rd.springer.com/article/10.1007%2Fs11033-010-0092-4 10.1007/s11033-010-0092-4
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institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English
description	Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the primary and amplified cDNA libraries were 1.1×10(6) and 6.0×10(9) pfu/ml respectively. The percentage of recombinants was 97% in the primary library and a total of 337 out of 415 original cDNA clones selected randomly contained inserts ranging from 600 to 1,500 bps. A total of 201 individual ESTs with sizes ranging from 390 to 1,038 bps were then analyzed and the BLASTX score revealed that 35.8% of the sequences were classified as strong match, 38.3% as nominal and 25.9% as weak match. Among the ESTs with known putative function, 21.4% of them were found to be related to gene expression, 14.4% ESTs to photosynthesis, 10.9% ESTs to metabolism, 5.5% ESTs to miscellaneous, 2.0% to stress response, and the remaining 45.8% were classified as novel genes. Analysis of ESTs described in this paper can be an effective approach to isolate and characterize new genes from A. convolutus and thus the sequences obtained represented a significant contribution to the extensive database of sequences from green microalgae.
format	Article
author	Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi
spellingShingle	Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
author_facet	Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi
author_sort	Thanh, Tran
title	Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
title_short	Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
title_full	Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
title_fullStr	Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
title_full_unstemmed	Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
title_sort	construction of cdna library and preliminary analysis of expressed sequence tags from green microalga ankistrodesmus convolutus corda
publisher	Springer
publishDate	2011
url	http://psasir.upm.edu.my/id/eprint/25181/ http://psasir.upm.edu.my/id/eprint/25181/ http://psasir.upm.edu.my/id/eprint/25181/ http://psasir.upm.edu.my/id/eprint/25181/1/Construction%20of%20cDNA%20library%20and%20preliminary%20analysis%20of%20expressed%20sequence%20tags%20from%20green%20microalga%20Ankistrodesmus%20convolutus%20Corda.pdf
first_indexed	2018-09-07T14:55:15Z
last_indexed	2018-09-07T14:55:15Z
_version_	1610960984844795904

Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda

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