Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique

Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique

This study was carried out to evaluate the potential of using the encapsulation-dehydration technique on cryopreservation protocorm-like bodies (PLBs) of Dendrobium sonia-17. The survival of the PLBs was assessed based on the effects of 4 dehydration periods (0, 1, 3 and 5 h) and 4 different concent...

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Main Authors: Subramaniam, Sreeramanan, Sinniah, Uma Rani, Khoddamzadeh, Amir Ali, Periasamy, Suranthran, James Antony, Jessica Jeyanthi
Format: Article
Language:English
Published: Academic Journals 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23682/
http://psasir.upm.edu.my/id/eprint/23682/
http://psasir.upm.edu.my/id/eprint/23682/
http://psasir.upm.edu.my/id/eprint/23682/1/23682.pdf
id upm-23682
recordtype eprints
spelling upm-236822016-05-31T07:55:19Z http://psasir.upm.edu.my/id/eprint/23682/ Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique Subramaniam, Sreeramanan Sinniah, Uma Rani Khoddamzadeh, Amir Ali Periasamy, Suranthran James Antony, Jessica Jeyanthi This study was carried out to evaluate the potential of using the encapsulation-dehydration technique on cryopreservation protocorm-like bodies (PLBs) of Dendrobium sonia-17. The survival of the PLBs was assessed based on the effects of 4 dehydration periods (0, 1, 3 and 5 h) and 4 different concentrations of 24-h sucrose pretreatment (0, 0.3, 0.5 and 0.7 M). Upon dehydration, moisture content was determined and the PLBs were evaluated for survival using absorbance values from 2, 3,5- triphenyltetrazolium chloride (TTC) assay at 530 nm and regeneration observations. Moisture content declined with the dehydration time, but the decline was not significant for encapsulated PLBs. All cryopreserved PLBs gave very low survival irrespective of the dehydration period. The best survival percentage in the cryopreservation of the PLBs of Dendrobium sonia-17 was obtained when the combination of 0.5 M sucrose pretreatment and 3 h dehydration time was applied in the experiment. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23682/1/23682.pdf Subramaniam, Sreeramanan and Sinniah, Uma Rani and Khoddamzadeh, Amir Ali and Periasamy, Suranthran and James Antony, Jessica Jeyanthi (2011) Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique. African Journal of Biotechnology, 10 (19). art. no. 17DD02527764. pp. 3902-3907. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/17DD02527764 10.5897/AJB10.2265
repository_type Digital Repository
institution_category Local University
institution Universiti Putra Malaysia
building UPM Institutional Repository
collection Online Access
language English
description This study was carried out to evaluate the potential of using the encapsulation-dehydration technique on cryopreservation protocorm-like bodies (PLBs) of Dendrobium sonia-17. The survival of the PLBs was assessed based on the effects of 4 dehydration periods (0, 1, 3 and 5 h) and 4 different concentrations of 24-h sucrose pretreatment (0, 0.3, 0.5 and 0.7 M). Upon dehydration, moisture content was determined and the PLBs were evaluated for survival using absorbance values from 2, 3,5- triphenyltetrazolium chloride (TTC) assay at 530 nm and regeneration observations. Moisture content declined with the dehydration time, but the decline was not significant for encapsulated PLBs. All cryopreserved PLBs gave very low survival irrespective of the dehydration period. The best survival percentage in the cryopreservation of the PLBs of Dendrobium sonia-17 was obtained when the combination of 0.5 M sucrose pretreatment and 3 h dehydration time was applied in the experiment.
format Article
author Subramaniam, Sreeramanan
Sinniah, Uma Rani
Khoddamzadeh, Amir Ali
Periasamy, Suranthran
James Antony, Jessica Jeyanthi
spellingShingle Subramaniam, Sreeramanan
Sinniah, Uma Rani
Khoddamzadeh, Amir Ali
Periasamy, Suranthran
James Antony, Jessica Jeyanthi
Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
author_facet Subramaniam, Sreeramanan
Sinniah, Uma Rani
Khoddamzadeh, Amir Ali
Periasamy, Suranthran
James Antony, Jessica Jeyanthi
author_sort Subramaniam, Sreeramanan
title Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
title_short Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
title_full Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
title_fullStr Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
title_full_unstemmed Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
title_sort fundamental concept of cryopreservation using dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
publisher Academic Journals
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/23682/
http://psasir.upm.edu.my/id/eprint/23682/
http://psasir.upm.edu.my/id/eprint/23682/
http://psasir.upm.edu.my/id/eprint/23682/1/23682.pdf
first_indexed 2018-09-07T14:41:51Z
last_indexed 2018-09-07T14:41:51Z
_version_ 1610960141071417344

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