Optimum conditions for the production of lipase by alginate-immobilized bacteria

A lipolytic Pseudomonas sp. has been successfully immobilized in strontium alginate gel bead for use in the production of lipase. This paper reports on the various conditions required for its optimum production. A 3% alginate gel concentration with a 20% (w/v) cell loading gives the highest producti...

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Main Authors:	Zakaria, Zainoha, Abdul Razak, Che Nyonya, Ampon, Kamaruzaman, Basri, Mahiran, Wan Yunus, Wan Md. Zin, Shirai, Yoshihito, Salleh, Abu Bakar, Hashimoto, Kenji
Format:	Article
Language:	English
Published:	Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1992
Online Access:	http://psasir.upm.edu.my/id/eprint/22565/ http://psasir.upm.edu.my/id/eprint/22565/ http://psasir.upm.edu.my/id/eprint/22565/ http://psasir.upm.edu.my/id/eprint/22565/1/Optimum%20conditions%20for%20the%20production%20of%20lipase%20by%20alginate-immobilized%20bacteria.pdf

id	upm-22565
recordtype	eprints
spelling	upm-225652016-04-25T04:48:05Z http://psasir.upm.edu.my/id/eprint/22565/ Optimum conditions for the production of lipase by alginate-immobilized bacteria Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji A lipolytic Pseudomonas sp. has been successfully immobilized in strontium alginate gel bead for use in the production of lipase. This paper reports on the various conditions required for its optimum production. A 3% alginate gel concentration with a 20% (w/v) cell loading gives the highest production of lipase. The production of lipase can be enhanced by aeration and shaking but mass transfer effect may be dependent on the ratio of cell mass and bead size. Prolonged recycling with aeration accelerates the rupturing of the beads compared to non-aerated system. A maximum production of lipase is given by 1.5g of immobilized bacteria in 50ml of culture broth. The immobilized bacteria can withstand recycling up to 24 days, (72h cycle), after which time the beads ruptured. The production, however, remains at 70%. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1992 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22565/1/Optimum%20conditions%20for%20the%20production%20of%20lipase%20by%20alginate-immobilized%20bacteria.pdf Zakaria, Zainoha and Abdul Razak, Che Nyonya and Ampon, Kamaruzaman and Basri, Mahiran and Wan Yunus, Wan Md. Zin and Shirai, Yoshihito and Salleh, Abu Bakar and Hashimoto, Kenji (1992) Optimum conditions for the production of lipase by alginate-immobilized bacteria. Journal of General and Applied Microbiology, 38 (5). pp. 429-438. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam1955/38/5/38_5_429/_article 10.2323/jgam.38.429
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English
description	A lipolytic Pseudomonas sp. has been successfully immobilized in strontium alginate gel bead for use in the production of lipase. This paper reports on the various conditions required for its optimum production. A 3% alginate gel concentration with a 20% (w/v) cell loading gives the highest production of lipase. The production of lipase can be enhanced by aeration and shaking but mass transfer effect may be dependent on the ratio of cell mass and bead size. Prolonged recycling with aeration accelerates the rupturing of the beads compared to non-aerated system. A maximum production of lipase is given by 1.5g of immobilized bacteria in 50ml of culture broth. The immobilized bacteria can withstand recycling up to 24 days, (72h cycle), after which time the beads ruptured. The production, however, remains at 70%.
format	Article
author	Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji
spellingShingle	Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji Optimum conditions for the production of lipase by alginate-immobilized bacteria
author_facet	Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji
author_sort	Zakaria, Zainoha
title	Optimum conditions for the production of lipase by alginate-immobilized bacteria
title_short	Optimum conditions for the production of lipase by alginate-immobilized bacteria
title_full	Optimum conditions for the production of lipase by alginate-immobilized bacteria
title_fullStr	Optimum conditions for the production of lipase by alginate-immobilized bacteria
title_full_unstemmed	Optimum conditions for the production of lipase by alginate-immobilized bacteria
title_sort	optimum conditions for the production of lipase by alginate-immobilized bacteria
publisher	Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
publishDate	1992
url	http://psasir.upm.edu.my/id/eprint/22565/ http://psasir.upm.edu.my/id/eprint/22565/ http://psasir.upm.edu.my/id/eprint/22565/ http://psasir.upm.edu.my/id/eprint/22565/1/Optimum%20conditions%20for%20the%20production%20of%20lipase%20by%20alginate-immobilized%20bacteria.pdf
first_indexed	2018-09-07T14:33:39Z
last_indexed	2018-09-07T14:33:39Z
_version_	1610959625174122496

Optimum conditions for the production of lipase by alginate-immobilized bacteria

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