Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts

The present study was conducted to determine the phytochemical compounds in different solvent extracts of Jatropha curcas Linn. plant and antibacterial activity of crude extracts. Aqueous, methanolic and hexane extracts of various plant parts were analysed for phytochemical compounds by spectrophoto...

Full description

Bibliographic Details
Main Authors:	Aidah, Namuli, Abdullah, Norhani, Sieo, Chin Chin, Saad, Wan Zuhainis, Oskoueian, Ehsan
Format:	Article
Language:	English
Published:	Academic Journals 2011
Online Access:	http://psasir.upm.edu.my/id/eprint/22261/ http://psasir.upm.edu.my/id/eprint/22261/ http://psasir.upm.edu.my/id/eprint/22261/1/Phytochemical%20compounds%20and%20antibacterial%20activity%20of%20Jatropha%20curcas%20Linn.%20extracts.pdf

id	upm-22261
recordtype	eprints
spelling	upm-222612016-08-23T05:46:09Z http://psasir.upm.edu.my/id/eprint/22261/ Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts Aidah, Namuli Abdullah, Norhani Sieo, Chin Chin Saad, Wan Zuhainis Oskoueian, Ehsan The present study was conducted to determine the phytochemical compounds in different solvent extracts of Jatropha curcas Linn. plant and antibacterial activity of crude extracts. Aqueous, methanolic and hexane extracts of various plant parts were analysed for phytochemical compounds by spectrophotometry, high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry analysis (GC-MS). Antibacterial activity was studied by paper disc diffusion assay against Gram positive and Gram negative bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by micro-broth dilution. The root bark methanolic extract contained high phenolics (11.51 mg gallic acid equivalents/g DW) and flavonoids (0.94 mg rutin equivalents/g DW). Kernel meal aqueous extract contained high saponins (0.65 mg diosgenin equivalents/g DW) and the methanolic extract contained 1.13 mg/g DW phorbol esters. Phytochemicals detected by RP-HPLC were pyrogallol, gallic acid, naringin, rutin and vanillic acid. The main compounds detected by GC-MS were oxalic acid (root bark), acetic acid and oleic acid (stem bark). Inhibition zones ranged from 8.0 to 17.7 mm. Low MIC (1.2 to 2.3 mg/ml) and MBC (0.4 to 6.3 mg/ml) values were observed in methanolic extract of all plant parts. The present study showed that stem bark, root bark and kernel meal of J. curcas contained compounds with antibacterial activities. The results indicate the potential of J. curcas as a source of antibacterial compounds. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22261/1/Phytochemical%20compounds%20and%20antibacterial%20activity%20of%20Jatropha%20curcas%20Linn.%20extracts.pdf Aidah, Namuli and Abdullah, Norhani and Sieo, Chin Chin and Saad, Wan Zuhainis and Oskoueian, Ehsan (2011) Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts. Journal of Medicinal Plants Research, 5 (16). art. no. 7052DE322885. pp. 3982-3990. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/article-abstract/7052DE322885
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English
description	The present study was conducted to determine the phytochemical compounds in different solvent extracts of Jatropha curcas Linn. plant and antibacterial activity of crude extracts. Aqueous, methanolic and hexane extracts of various plant parts were analysed for phytochemical compounds by spectrophotometry, high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry analysis (GC-MS). Antibacterial activity was studied by paper disc diffusion assay against Gram positive and Gram negative bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by micro-broth dilution. The root bark methanolic extract contained high phenolics (11.51 mg gallic acid equivalents/g DW) and flavonoids (0.94 mg rutin equivalents/g DW). Kernel meal aqueous extract contained high saponins (0.65 mg diosgenin equivalents/g DW) and the methanolic extract contained 1.13 mg/g DW phorbol esters. Phytochemicals detected by RP-HPLC were pyrogallol, gallic acid, naringin, rutin and vanillic acid. The main compounds detected by GC-MS were oxalic acid (root bark), acetic acid and oleic acid (stem bark). Inhibition zones ranged from 8.0 to 17.7 mm. Low MIC (1.2 to 2.3 mg/ml) and MBC (0.4 to 6.3 mg/ml) values were observed in methanolic extract of all plant parts. The present study showed that stem bark, root bark and kernel meal of J. curcas contained compounds with antibacterial activities. The results indicate the potential of J. curcas as a source of antibacterial compounds.
format	Article
author	Aidah, Namuli Abdullah, Norhani Sieo, Chin Chin Saad, Wan Zuhainis Oskoueian, Ehsan
spellingShingle	Aidah, Namuli Abdullah, Norhani Sieo, Chin Chin Saad, Wan Zuhainis Oskoueian, Ehsan Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts
author_facet	Aidah, Namuli Abdullah, Norhani Sieo, Chin Chin Saad, Wan Zuhainis Oskoueian, Ehsan
author_sort	Aidah, Namuli
title	Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts
title_short	Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts
title_full	Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts
title_fullStr	Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts
title_full_unstemmed	Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts
title_sort	phytochemical compounds and antibacterial activity of jatropha curcas linn. extracts
publisher	Academic Journals
publishDate	2011
url	http://psasir.upm.edu.my/id/eprint/22261/ http://psasir.upm.edu.my/id/eprint/22261/ http://psasir.upm.edu.my/id/eprint/22261/1/Phytochemical%20compounds%20and%20antibacterial%20activity%20of%20Jatropha%20curcas%20Linn.%20extracts.pdf
first_indexed	2018-09-07T14:31:13Z
last_indexed	2018-09-07T14:31:13Z
_version_	1610959472263430144

Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts

Similar Items