Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides

Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spect...

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Main Authors:	Md. Yunus, Noor Mona, Abdul Rahman, Mohd Basyaruddin, Basri, Mahiran, Hussein, Mohd Zobir, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha
Format:	Conference or Workshop Item
Language:	English
Published:	2007
Online Access:	http://psasir.upm.edu.my/id/eprint/18994/ http://psasir.upm.edu.my/id/eprint/18994/1/ID%2018994.pdf

id	upm-18994
recordtype	eprints
spelling	upm-189942016-09-02T09:37:47Z http://psasir.upm.edu.my/id/eprint/18994/ Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides Md. Yunus, Noor Mona Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Hussein, Mohd Zobir Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spectroscopic and microscopic evidence for the adsorption of CRL onto CLDH. SEM-EDX spectra show CRL presence in CMAN-CRL, CNAN-CRL and CZAN-CRL prepared. Blue spots on the surface of CMAN-CRL,CNAN-CRL and CZAN-CRL stained with Coomasie Blue R confirm protein adsorption onto supports. The catalytic activity of all three CLDH-CRL was also evaluated in synthesis of methyl caprylate and results showed that CMAN-CRL and CNAN-CRL exhibited 70% conversion while CZAN-CRL exhibited 80% conversion compared to free lipase that exhibited only 6% conversion only. 2007 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18994/1/ID%2018994.pdf Md. Yunus, Noor Mona and Abdul Rahman, Mohd Basyaruddin and Basri, Mahiran and Hussein, Mohd Zobir and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2007) Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides. In: Enzyme Engineering XIX, 23-28 Sept. 2007, Columbia, Canada. .
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English
description	Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spectroscopic and microscopic evidence for the adsorption of CRL onto CLDH. SEM-EDX spectra show CRL presence in CMAN-CRL, CNAN-CRL and CZAN-CRL prepared. Blue spots on the surface of CMAN-CRL,CNAN-CRL and CZAN-CRL stained with Coomasie Blue R confirm protein adsorption onto supports. The catalytic activity of all three CLDH-CRL was also evaluated in synthesis of methyl caprylate and results showed that CMAN-CRL and CNAN-CRL exhibited 70% conversion while CZAN-CRL exhibited 80% conversion compared to free lipase that exhibited only 6% conversion only.
format	Conference or Workshop Item
author	Md. Yunus, Noor Mona Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Hussein, Mohd Zobir Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha
spellingShingle	Md. Yunus, Noor Mona Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Hussein, Mohd Zobir Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
author_facet	Md. Yunus, Noor Mona Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Hussein, Mohd Zobir Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha
author_sort	Md. Yunus, Noor Mona
title	Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
title_short	Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
title_full	Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
title_fullStr	Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
title_full_unstemmed	Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
title_sort	spectroscopic and microscopic evidence for adsorption of lipase from candida rugosa onto calcined layered double hydroxides
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/18994/ http://psasir.upm.edu.my/id/eprint/18994/1/ID%2018994.pdf
first_indexed	2018-09-07T14:06:29Z
last_indexed	2018-09-07T14:06:29Z
_version_	1610957916504850432

Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides

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