Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying

Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 m...

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Main Authors:	Abd Manap, Mohd Yazid, Shobirin, Anis, Mohamad, Rosfarizan, Mustafa, Shuhaimi, Wong, Stephenie, Kabeir, Barka Mohammed
Format:	Article
Language:	English English
Published:	Libertas Academica Ltd. 2009
Online Access:	http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/1/Viability%20of%20bifidobacterium%20pseudocatenulatum%20G4%20after%20spray.pdf

id	upm-17753
recordtype	eprints
spelling	upm-177532015-11-30T07:31:25Z http://psasir.upm.edu.my/id/eprint/17753/ Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%. Libertas Academica Ltd. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17753/1/Viability%20of%20bifidobacterium%20pseudocatenulatum%20G4%20after%20spray.pdf Abd Manap, Mohd Yazid and Shobirin, Anis and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Wong, Stephenie and Kabeir, Barka Mohammed (2009) Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying. Microbiology Insights, 2 (July). pp. 1-7. ISSN 1178-6361 10.4137/MBI.S2728 English
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English English
description	Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%.
format	Article
author	Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed
spellingShingle	Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
author_facet	Abd Manap, Mohd Yazid Shobirin, Anis Mohamad, Rosfarizan Mustafa, Shuhaimi Wong, Stephenie Kabeir, Barka Mohammed
author_sort	Abd Manap, Mohd Yazid
title	Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
title_short	Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
title_full	Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
title_fullStr	Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
title_full_unstemmed	Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying
title_sort	viability of bifidobacterium pseudocatenulatum g4 after spray-drying and freeze-drying
publisher	Libertas Academica Ltd.
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/ http://psasir.upm.edu.my/id/eprint/17753/1/Viability%20of%20bifidobacterium%20pseudocatenulatum%20G4%20after%20spray.pdf
first_indexed	2018-09-07T13:56:53Z
last_indexed	2018-09-07T13:56:53Z
_version_	1610957312691798016

Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying

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