Morphological alteration in mitochondria following diclofenac and ibuprofen administration.

This study was conducted to identify and to compare the mitochondrial morphological alterations in livers of rats treated with various doses of diclofenac and ibuprofen. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intrape...

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Main Authors:	Moorthy, Mohanambal, Fakurazi, Sharida, Ithnin, Hairuszah
Format:	Article
Language:	English English
Published:	Asian Network for Scientific Information 2008
Online Access:	http://psasir.upm.edu.my/id/eprint/15731/ http://psasir.upm.edu.my/id/eprint/15731/ http://psasir.upm.edu.my/id/eprint/15731/ http://psasir.upm.edu.my/id/eprint/15731/1/Morphological%20alteration%20in%20mitochondria%20following%20diclofenac%20and%20ibuprofen%20administration.pdf

id	upm-15731
recordtype	eprints
spelling	upm-157312015-10-26T00:03:43Z http://psasir.upm.edu.my/id/eprint/15731/ Morphological alteration in mitochondria following diclofenac and ibuprofen administration. Moorthy, Mohanambal Fakurazi, Sharida Ithnin, Hairuszah This study was conducted to identify and to compare the mitochondrial morphological alterations in livers of rats treated with various doses of diclofenac and ibuprofen. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intraperitoneal injection for 15 days. The control group was administered with saline in a similar manner. Four rats were euthanised every 3 days until day 15. While 200 mg kg-1 diclofenac and ibuprofen-treated rats (n = 4) were euthanized 10 h post-treatment. The livers were removed, cleaned and a section across the right lobe was taken and fixed in 4% (v/v) glutaraldehyde for electron microscopy analysis and the remaining samples were kept at -80°C for Western blot analysis. Five milligram per kilogram and 10 mg kg-1 diclofenac-administered rats for 15 days revealed the presence of enlarged mitochondria, irregular and ruptured mitochondrial membranes. While rats administered with 10 mg kg-1 ibuprofen also showed the presence of mitochondria with irregular membrane structure and ruptured membranes. Western blotting analysis of mitochondrial fractions revealed the expression of cytochrome c in all samples and complete absence of cytochrome c expression in the cytosolic fraction of all samples after day 15. Analysis in 200 mg kg -1 diclofenac and ibuprofen-treated groups, revealed expression of cytochrome c in both mitochondrial and cytosolic fractions. This observation indicates that both diclofenac and ibuprofen may alter the morphology of mitochondria, leading to cytochrome c release into the cytosol. Further studies needs to be conducted to investigate on the activity of the mitochondria following both treatments. Asian Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15731/1/Morphological%20alteration%20in%20mitochondria%20following%20diclofenac%20and%20ibuprofen%20administration.pdf Moorthy, Mohanambal and Fakurazi, Sharida and Ithnin, Hairuszah (2008) Morphological alteration in mitochondria following diclofenac and ibuprofen administration. Pakistan Journal of Biological Sciences, 11 (15). pp. 1901-1908. ISSN 1028-8880; ESSN: 1812-5735 http://www.ansinet.com/ 10.3923/pjbs.2008.1901.1908 English
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
language	English English
description	This study was conducted to identify and to compare the mitochondrial morphological alterations in livers of rats treated with various doses of diclofenac and ibuprofen. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intraperitoneal injection for 15 days. The control group was administered with saline in a similar manner. Four rats were euthanised every 3 days until day 15. While 200 mg kg-1 diclofenac and ibuprofen-treated rats (n = 4) were euthanized 10 h post-treatment. The livers were removed, cleaned and a section across the right lobe was taken and fixed in 4% (v/v) glutaraldehyde for electron microscopy analysis and the remaining samples were kept at -80°C for Western blot analysis. Five milligram per kilogram and 10 mg kg-1 diclofenac-administered rats for 15 days revealed the presence of enlarged mitochondria, irregular and ruptured mitochondrial membranes. While rats administered with 10 mg kg-1 ibuprofen also showed the presence of mitochondria with irregular membrane structure and ruptured membranes. Western blotting analysis of mitochondrial fractions revealed the expression of cytochrome c in all samples and complete absence of cytochrome c expression in the cytosolic fraction of all samples after day 15. Analysis in 200 mg kg -1 diclofenac and ibuprofen-treated groups, revealed expression of cytochrome c in both mitochondrial and cytosolic fractions. This observation indicates that both diclofenac and ibuprofen may alter the morphology of mitochondria, leading to cytochrome c release into the cytosol. Further studies needs to be conducted to investigate on the activity of the mitochondria following both treatments.
format	Article
author	Moorthy, Mohanambal Fakurazi, Sharida Ithnin, Hairuszah
spellingShingle	Moorthy, Mohanambal Fakurazi, Sharida Ithnin, Hairuszah Morphological alteration in mitochondria following diclofenac and ibuprofen administration.
author_facet	Moorthy, Mohanambal Fakurazi, Sharida Ithnin, Hairuszah
author_sort	Moorthy, Mohanambal
title	Morphological alteration in mitochondria following diclofenac and ibuprofen administration.
title_short	Morphological alteration in mitochondria following diclofenac and ibuprofen administration.
title_full	Morphological alteration in mitochondria following diclofenac and ibuprofen administration.
title_fullStr	Morphological alteration in mitochondria following diclofenac and ibuprofen administration.
title_full_unstemmed	Morphological alteration in mitochondria following diclofenac and ibuprofen administration.
title_sort	morphological alteration in mitochondria following diclofenac and ibuprofen administration.
publisher	Asian Network for Scientific Information
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/15731/ http://psasir.upm.edu.my/id/eprint/15731/ http://psasir.upm.edu.my/id/eprint/15731/ http://psasir.upm.edu.my/id/eprint/15731/1/Morphological%20alteration%20in%20mitochondria%20following%20diclofenac%20and%20ibuprofen%20administration.pdf
first_indexed	2018-09-07T13:39:11Z
last_indexed	2018-09-07T13:39:11Z
_version_	1610956199108280320

Morphological alteration in mitochondria following diclofenac and ibuprofen administration.

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