Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont

An experiment was carried out in order to study the effect of treatment containing 0.5 and 1.0 mg/L 6-benzylaminopurine (BAP) and effect of subculture on percentage of shoot formation, number of shoot per explant and mean shoot height of shoot tip explant of Psidium guajava L. var. Beaumont. MS medi...

Full description

Bibliographic Details
Main Authors:	Abdullah, Tajul Afif, Abdul Aziz, Maheran, Abdul Rashid, Azmi, Saleh, Ghizan, Elhory, Suleiman Mohamed Ahmed, Panjaitan, Syaiful Bahri, Sajili, Mohammad Hailmi, Mohamad, Noor Muzamil
Format:	Article
Published:	Institute of Biotechnology and Genetic Engineering 2009
Online Access:	http://psasir.upm.edu.my/id/eprint/13713/ http://psasir.upm.edu.my/id/eprint/13713/

id	upm-13713
recordtype	eprints
spelling	upm-137132016-01-18T08:33:19Z http://psasir.upm.edu.my/id/eprint/13713/ Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont Abdullah, Tajul Afif Abdul Aziz, Maheran Abdul Rashid, Azmi Saleh, Ghizan Elhory, Suleiman Mohamed Ahmed Panjaitan, Syaiful Bahri Sajili, Mohammad Hailmi Mohamad, Noor Muzamil An experiment was carried out in order to study the effect of treatment containing 0.5 and 1.0 mg/L 6-benzylaminopurine (BAP) and effect of subculture on percentage of shoot formation, number of shoot per explant and mean shoot height of shoot tip explant of Psidium guajava L. var. Beaumont. MS medium (Murashige and Skoog, 1962) was used with 30 g/L of sucrose and 7 g/L agar type 900. Shoot tips of elongated shoots were decapitated to abolish its apical dominance prior to sub-culturing was carried out to a fresh medium every 4 weeks. The increase in mean number of shoots per explant and the shoot height was observed for every subculture. Treatment 1.0 mg/L BAP in comparison to treatment 0.5 mg/L BAP showed higher mean number of shoot per explant per subculture whereas treatment 0.5 mg/L BAP showed higher mean shoot height per subculture. Data were analyzed using T-test until the fifth subculture. Institute of Biotechnology and Genetic Engineering 2009 Article PeerReviewed Abdullah, Tajul Afif and Abdul Aziz, Maheran and Abdul Rashid, Azmi and Saleh, Ghizan and Elhory, Suleiman Mohamed Ahmed and Panjaitan, Syaiful Bahri and Sajili, Mohammad Hailmi and Mohamad, Noor Muzamil (2009) Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont. Pakistan Journal of Biotechnology, 6 (1-2). pp. 21-26. ISSN 1812-1837; ESSN: 2312-7791 http://pjbt.org/pakistan-journal-of-biotechnology-vol-6-no-1-2-of-2009/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Putra Malaysia
building	UPM Institutional Repository
collection	Online Access
description	An experiment was carried out in order to study the effect of treatment containing 0.5 and 1.0 mg/L 6-benzylaminopurine (BAP) and effect of subculture on percentage of shoot formation, number of shoot per explant and mean shoot height of shoot tip explant of Psidium guajava L. var. Beaumont. MS medium (Murashige and Skoog, 1962) was used with 30 g/L of sucrose and 7 g/L agar type 900. Shoot tips of elongated shoots were decapitated to abolish its apical dominance prior to sub-culturing was carried out to a fresh medium every 4 weeks. The increase in mean number of shoots per explant and the shoot height was observed for every subculture. Treatment 1.0 mg/L BAP in comparison to treatment 0.5 mg/L BAP showed higher mean number of shoot per explant per subculture whereas treatment 0.5 mg/L BAP showed higher mean shoot height per subculture. Data were analyzed using T-test until the fifth subculture.
format	Article
author	Abdullah, Tajul Afif Abdul Aziz, Maheran Abdul Rashid, Azmi Saleh, Ghizan Elhory, Suleiman Mohamed Ahmed Panjaitan, Syaiful Bahri Sajili, Mohammad Hailmi Mohamad, Noor Muzamil
spellingShingle	Abdullah, Tajul Afif Abdul Aziz, Maheran Abdul Rashid, Azmi Saleh, Ghizan Elhory, Suleiman Mohamed Ahmed Panjaitan, Syaiful Bahri Sajili, Mohammad Hailmi Mohamad, Noor Muzamil Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont
author_facet	Abdullah, Tajul Afif Abdul Aziz, Maheran Abdul Rashid, Azmi Saleh, Ghizan Elhory, Suleiman Mohamed Ahmed Panjaitan, Syaiful Bahri Sajili, Mohammad Hailmi Mohamad, Noor Muzamil
author_sort	Abdullah, Tajul Afif
title	Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont
title_short	Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont
title_full	Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont
title_fullStr	Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont
title_full_unstemmed	Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont
title_sort	comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of psidium guajava l. var. beaumont
publisher	Institute of Biotechnology and Genetic Engineering
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/13713/ http://psasir.upm.edu.my/id/eprint/13713/
first_indexed	2018-09-07T13:25:39Z
last_indexed	2018-09-07T13:25:39Z
_version_	1610955347089948672

Comparison on the effect of treatment and subculturing on shoot regeneration from shoot tip seedlings of Psidium guajava L. var. Beaumont

Similar Items