Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer

Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer

The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of...

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Main Author: Suliana, Charles Nyucong
Format: Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, UNIMAS 2008
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/7655/
http://ir.unimas.my/7655/1/Evaluation%20of%20Genetic%20Relatedness%20Among%20Acacia%20Superbulk%20and%20Acacia%20Hybrid%20%28Acacia%20Mangium%20X%20Acacia%20Auriculiformis%29%20Trees%20Using%20M13%20Universal%20Primer%2024%20pgs.pdf
http://ir.unimas.my/7655/2/Evaluation%20of%20Genetic%20Relatedness%20Among%20Acacia%20Superbulk%20and%20Acacia%20Hybrid%20%28Acacia%20Mangium%20X%20Acacia%20Auriculiformis%29%20Trees%20Using%20M13%20Universal%20Primer.pdf
id unimas-7655
recordtype eprints
spelling unimas-76552015-06-18T07:02:38Z http://ir.unimas.my/7655/ Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer Suliana, Charles Nyucong Q Science (General) The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of the fragments were identified as polymorphic band with the size ranging from 500bp to 2.5kbp. An UPGMA dendrogram was constructed based on the Jaccard’s coefficient of similarity matrix which then grouped the Acacia superbulk and Acacia hybrid into two main clusters, i.e. Cluster 1: Acacia superbulk trees and Cluster 2: Acacia hybrid trees. All Acacia hybrid trees showed identical banding profile and therefore, suggested that these trees were originated from the same clone, whereas Acacia superbulk trees were originated from randomly selected plus trees seeds of A. mangium. The preliminary results showed that M13 universal primer is a potential molecular marker not only for assessing genetic relatedness but also could be used for clonal identification and seedling certification. However, further study by using other DAMD markers such as YNZ-22 and 33.6 can be carried out to validate these results. Universiti Malaysia Sarawak, UNIMAS 2008 Project Report NonPeerReviewed text en http://ir.unimas.my/7655/1/Evaluation%20of%20Genetic%20Relatedness%20Among%20Acacia%20Superbulk%20and%20Acacia%20Hybrid%20%28Acacia%20Mangium%20X%20Acacia%20Auriculiformis%29%20Trees%20Using%20M13%20Universal%20Primer%2024%20pgs.pdf text en http://ir.unimas.my/7655/2/Evaluation%20of%20Genetic%20Relatedness%20Among%20Acacia%20Superbulk%20and%20Acacia%20Hybrid%20%28Acacia%20Mangium%20X%20Acacia%20Auriculiformis%29%20Trees%20Using%20M13%20Universal%20Primer.pdf Suliana, Charles Nyucong (2008) Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer. [Project Report] (Unpublished)
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Sarawak
building UNIMAS Institutional Repository
collection Online Access
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Suliana, Charles Nyucong
Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
description The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of the fragments were identified as polymorphic band with the size ranging from 500bp to 2.5kbp. An UPGMA dendrogram was constructed based on the Jaccard’s coefficient of similarity matrix which then grouped the Acacia superbulk and Acacia hybrid into two main clusters, i.e. Cluster 1: Acacia superbulk trees and Cluster 2: Acacia hybrid trees. All Acacia hybrid trees showed identical banding profile and therefore, suggested that these trees were originated from the same clone, whereas Acacia superbulk trees were originated from randomly selected plus trees seeds of A. mangium. The preliminary results showed that M13 universal primer is a potential molecular marker not only for assessing genetic relatedness but also could be used for clonal identification and seedling certification. However, further study by using other DAMD markers such as YNZ-22 and 33.6 can be carried out to validate these results.
format Project Report
author Suliana, Charles Nyucong
author_facet Suliana, Charles Nyucong
author_sort Suliana, Charles Nyucong
title Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
title_short Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
title_full Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
title_fullStr Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
title_full_unstemmed Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
title_sort evaluation of genetic relatedness among acacia superbulk and acacia hybrid (acacia mangium x acacia auriculiformis) trees using m13 universal primer
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2008
url http://ir.unimas.my/7655/
http://ir.unimas.my/7655/1/Evaluation%20of%20Genetic%20Relatedness%20Among%20Acacia%20Superbulk%20and%20Acacia%20Hybrid%20%28Acacia%20Mangium%20X%20Acacia%20Auriculiformis%29%20Trees%20Using%20M13%20Universal%20Primer%2024%20pgs.pdf
http://ir.unimas.my/7655/2/Evaluation%20of%20Genetic%20Relatedness%20Among%20Acacia%20Superbulk%20and%20Acacia%20Hybrid%20%28Acacia%20Mangium%20X%20Acacia%20Auriculiformis%29%20Trees%20Using%20M13%20Universal%20Primer.pdf
first_indexed 2018-09-06T15:28:07Z
last_indexed 2018-09-06T15:28:07Z
_version_ 1610872455114522624

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