DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri

Borneo Ironwood, consists of two species, i.e. Eusideroxylon zwageri and Potoxylon melagangai, is the most well known durable tropical hardwood timber tree of Borneo. Both species are morphologically similar, except the wood characteristics. This has resulted in taxonomic difference between them. W...

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Main Authors:	Ho Wei, Seng, Yii Ai, Siew, Lee Shiat, Mei, Cheksum, Tawan, Isa, Ipor, Pang Shek, Ling, Julaihi, Abdullah
Format:	Article
Language:	English
Published:	Ecological Association of Malaysia 2014
Subjects:	Q Science (General) SD Forestry
Online Access:	http://ir.unimas.my/7302/ http://ir.unimas.my/7302/ http://ir.unimas.my/7302/1/DNA%20Genotyping%20of%20Borneo.pdf

id	unimas-7302
recordtype	eprints
spelling	unimas-73022015-09-11T00:52:11Z http://ir.unimas.my/7302/ DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri Ho Wei, Seng Yii Ai, Siew Lee Shiat, Mei Cheksum, Tawan Isa, Ipor Pang Shek, Ling Julaihi, Abdullah Q Science (General) SD Forestry Borneo Ironwood, consists of two species, i.e. Eusideroxylon zwageri and Potoxylon melagangai, is the most well known durable tropical hardwood timber tree of Borneo. Both species are morphologically similar, except the wood characteristics. This has resulted in taxonomic difference between them. We used PCR with M13 universal primer to determine the genetic relatedness and SCAR marker to distinguish both species. Cluster analysis grouped both species into two major clusters, suggesting that there are two genetically distantly related species. One diagnostic band (locus M13-16) was successfully converted to SCAR marker. A single fragment (720 bp) was amplified in all E. zwageri individuals but completely absent in P. melagangai. Differentiating the species by SCAR marker is very useful for screening large numbers of Borneo Ironwood seedlings collected from natural populations. The identification of individuals at species level constitutes one of the first basics in any effective conservation programme. In fact, the Borneo Ironwood has been counted as one of the endangered species in Sarawak. Ecological Association of Malaysia 2014 Article PeerReviewed text en http://ir.unimas.my/7302/1/DNA%20Genotyping%20of%20Borneo.pdf Ho Wei, Seng and Yii Ai, Siew and Lee Shiat, Mei and Cheksum, Tawan and Isa, Ipor and Pang Shek, Ling and Julaihi, Abdullah (2014) DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri. Acta Biologica Malaysiana, 3 (1). pp. 10-15. ISSN 2232-1675 http://www.science.upm.edu.my/biology/abm/images/pdf/vol3pp10-15.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) SD Forestry
spellingShingle	Q Science (General) SD Forestry Ho Wei, Seng Yii Ai, Siew Lee Shiat, Mei Cheksum, Tawan Isa, Ipor Pang Shek, Ling Julaihi, Abdullah DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri
description	Borneo Ironwood, consists of two species, i.e. Eusideroxylon zwageri and Potoxylon melagangai, is the most well known durable tropical hardwood timber tree of Borneo. Both species are morphologically similar, except the wood characteristics. This has resulted in taxonomic difference between them. We used PCR with M13 universal primer to determine the genetic relatedness and SCAR marker to distinguish both species. Cluster analysis grouped both species into two major clusters, suggesting that there are two genetically distantly related species. One diagnostic band (locus M13-16) was successfully converted to SCAR marker. A single fragment (720 bp) was amplified in all E. zwageri individuals but completely absent in P. melagangai. Differentiating the species by SCAR marker is very useful for screening large numbers of Borneo Ironwood seedlings collected from natural populations. The identification of individuals at species level constitutes one of the first basics in any effective conservation programme. In fact, the Borneo Ironwood has been counted as one of the endangered species in Sarawak.
format	Article
author	Ho Wei, Seng Yii Ai, Siew Lee Shiat, Mei Cheksum, Tawan Isa, Ipor Pang Shek, Ling Julaihi, Abdullah
author_facet	Ho Wei, Seng Yii Ai, Siew Lee Shiat, Mei Cheksum, Tawan Isa, Ipor Pang Shek, Ling Julaihi, Abdullah
author_sort	Ho Wei, Seng
title	DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri
title_short	DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri
title_full	DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri
title_fullStr	DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri
title_full_unstemmed	DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri
title_sort	dna genotyping of borneo ironwood using m13 universal primer and scar marker development in eusideroxylon zwageri
publisher	Ecological Association of Malaysia
publishDate	2014
url	http://ir.unimas.my/7302/ http://ir.unimas.my/7302/ http://ir.unimas.my/7302/1/DNA%20Genotyping%20of%20Borneo.pdf
first_indexed	2018-09-06T15:25:35Z
last_indexed	2018-09-06T15:25:35Z
_version_	1610872296351727616

DNA Genotyping of Borneo Ironwood using M13 Universal Primer and SCAR Marker Development in Eusideroxylon zwageri

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