Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population

Background: Colorectal cancer (CRC) is a multifactorial disease with factors including dietary and lifestyle habits and genetic predisposition contributing to its etiopathogenesis. Even though the genetic predisposing factors are still unclear, genetic polymorphisms of genes encoding enzymes involve...

Full description

Bibliographic Details
Main Authors:	Siti Nurfatimah, Mohd Shahpudin, Mohd Aminudin, Mustapha, Ahmad Aizat, Abdul Aziz, Murali Krishana, Bahavaraju Venkata, Chatar Singh, Gurjeet Kaur, Venkatesh R, Naik, Zaidi, Zakaria, Ahmad Shanwani, Mohd Sidek, Mohammad Radzi, Abu Hassan, Ankathil, Ravindran
Format:	Article
Language:	English
Published:	Japan International Cultural Exchange Foundation & Japan Health Sciences University 2011
Subjects:	Q Science (General) R Medicine (General)
Online Access:	http://ir.unimas.my/700/ http://ir.unimas.my/700/ http://ir.unimas.my/700/1/glutathione%20S%20transferase%20PI.pdf

id	unimas-700
recordtype	eprints
spelling	unimas-7002016-04-12T02:33:04Z http://ir.unimas.my/700/ Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population Siti Nurfatimah, Mohd Shahpudin Mohd Aminudin, Mustapha Ahmad Aizat, Abdul Aziz Murali Krishana, Bahavaraju Venkata Chatar Singh, Gurjeet Kaur Venkatesh R, Naik Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran Q Science (General) R Medicine (General) Background: Colorectal cancer (CRC) is a multifactorial disease with factors including dietary and lifestyle habits and genetic predisposition contributing to its etiopathogenesis. Even though the genetic predisposing factors are still unclear, genetic polymorphisms of genes encoding enzymes involved in xenobiotic metabolic pathways that activate or inactivate dietary carcinogens have been proposed as candidate genes. Three members of the Gluthathione S-Transferase (GSTs) family GSTPI, GSTT1 and GSTM1 have been analyzed in Malaysian population for polymorphic variants, and to elucidate their role in colorectal carcinogenesis. Objective: To determine the frequencies of GSTPI, GSTTI and GSTMI genotypes in 111 histopathologically confirmed CRC patients and 128 healthy controls and to evaluate the association risk of GSTPI, GSTTI and GSTMI genotypes on CRC predisposition. Material and Methods: Peripheral blood from the study subjects were collected in EDTA tubes and genomic DNA extracted using QIAGEN kit. The GSTPI IlelO5Val polymorphism was analyzed by PCR-RFLP technique using BsmAl restriction enzyme. The presence or absence of GSTM1 and GSTT1, genes were determined using a multiplex PCR protocol with albumin as the housekeeping gene. The resulting PCR fragments were separated on 2.0% agarose gel for GSTPI and 3.0% agarose gel electrophoresis for the GSTT1 and GSTM1. Results and Conclusion: On evaluating the CRC risk association with variant genotypes singly, GSTTI null genotype was associated significantly with an elevated risk (OR 1.804, 95%CI: 1.065-3.361, p=0027). When the polymorphic genotypes were analyzed in combination, the combination genotypes of GSTPI Val/Val/GSTM+/GSTTIÃ· (OR 4.000), Val/Val/GSTMI-/GSTTI- (OR 3.000), and GSTPI Ile/Ile/GSTMI+/GSTTI- (OR 2.833) showed higher associated risk for CRC susceptibility, however the risk values were not statistically significant. Further studies involving larger sample size may help to identify the more specific risk groups and to determine factors of importance in CRC development. Japan International Cultural Exchange Foundation & Japan Health Sciences University 2011-12 Article NonPeerReviewed text en http://ir.unimas.my/700/1/glutathione%20S%20transferase%20PI.pdf Siti Nurfatimah, Mohd Shahpudin and Mohd Aminudin, Mustapha and Ahmad Aizat, Abdul Aziz and Murali Krishana, Bahavaraju Venkata and Chatar Singh, Gurjeet Kaur and Venkatesh R, Naik and Zaidi, Zakaria and Ahmad Shanwani, Mohd Sidek and Mohammad Radzi, Abu Hassan and Ankathil, Ravindran (2011) Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population. International Medical Journal. , 18 (4). pp. 279-282. http://connection.ebscohost.com/c/articles/71093480/glutathione-s-transferase-p1-mi-ti-genotypes-risk-colorectal-cancer-development-malaysian-population
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) R Medicine (General)
spellingShingle	Q Science (General) R Medicine (General) Siti Nurfatimah, Mohd Shahpudin Mohd Aminudin, Mustapha Ahmad Aizat, Abdul Aziz Murali Krishana, Bahavaraju Venkata Chatar Singh, Gurjeet Kaur Venkatesh R, Naik Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population
description	Background: Colorectal cancer (CRC) is a multifactorial disease with factors including dietary and lifestyle habits and genetic predisposition contributing to its etiopathogenesis. Even though the genetic predisposing factors are still unclear, genetic polymorphisms of genes encoding enzymes involved in xenobiotic metabolic pathways that activate or inactivate dietary carcinogens have been proposed as candidate genes. Three members of the Gluthathione S-Transferase (GSTs) family GSTPI, GSTT1 and GSTM1 have been analyzed in Malaysian population for polymorphic variants, and to elucidate their role in colorectal carcinogenesis. Objective: To determine the frequencies of GSTPI, GSTTI and GSTMI genotypes in 111 histopathologically confirmed CRC patients and 128 healthy controls and to evaluate the association risk of GSTPI, GSTTI and GSTMI genotypes on CRC predisposition. Material and Methods: Peripheral blood from the study subjects were collected in EDTA tubes and genomic DNA extracted using QIAGEN kit. The GSTPI IlelO5Val polymorphism was analyzed by PCR-RFLP technique using BsmAl restriction enzyme. The presence or absence of GSTM1 and GSTT1, genes were determined using a multiplex PCR protocol with albumin as the housekeeping gene. The resulting PCR fragments were separated on 2.0% agarose gel for GSTPI and 3.0% agarose gel electrophoresis for the GSTT1 and GSTM1. Results and Conclusion: On evaluating the CRC risk association with variant genotypes singly, GSTTI null genotype was associated significantly with an elevated risk (OR 1.804, 95%CI: 1.065-3.361, p=0027). When the polymorphic genotypes were analyzed in combination, the combination genotypes of GSTPI Val/Val/GSTM+/GSTTIÃ· (OR 4.000), Val/Val/GSTMI-/GSTTI- (OR 3.000), and GSTPI Ile/Ile/GSTMI+/GSTTI- (OR 2.833) showed higher associated risk for CRC susceptibility, however the risk values were not statistically significant. Further studies involving larger sample size may help to identify the more specific risk groups and to determine factors of importance in CRC development.
format	Article
author	Siti Nurfatimah, Mohd Shahpudin Mohd Aminudin, Mustapha Ahmad Aizat, Abdul Aziz Murali Krishana, Bahavaraju Venkata Chatar Singh, Gurjeet Kaur Venkatesh R, Naik Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran
author_facet	Siti Nurfatimah, Mohd Shahpudin Mohd Aminudin, Mustapha Ahmad Aizat, Abdul Aziz Murali Krishana, Bahavaraju Venkata Chatar Singh, Gurjeet Kaur Venkatesh R, Naik Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hassan Ankathil, Ravindran
author_sort	Siti Nurfatimah, Mohd Shahpudin
title	Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population
title_short	Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population
title_full	Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population
title_fullStr	Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population
title_full_unstemmed	Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population
title_sort	glutathione s tranferase pi, mi and ti genotypes and risk for colorectal cancer development in malaysian population
publisher	Japan International Cultural Exchange Foundation & Japan Health Sciences University
publishDate	2011
url	http://ir.unimas.my/700/ http://ir.unimas.my/700/ http://ir.unimas.my/700/1/glutathione%20S%20transferase%20PI.pdf
first_indexed	2018-09-06T14:38:17Z
last_indexed	2018-09-06T14:38:17Z
_version_	1610869319720239104

Glutathione S tranferase PI, MI and TI genotypes and risk for colorectal cancer development in Malaysian population

Similar Items