Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)

Neolamarckia cadamba (Roxb.) Bosser, also known as Kelampayan, is a medium to large sized, fast growing tree that is belongs to the family of Rubiaceae. Kelampayan is used for industrial plantation and reforestation. It gives the best raw materials for plywood industry as well as pulp and paper indu...

Full description

Bibliographic Details
Main Author:	Ling, Siaw Ching.
Format:	Project Report
Language:	English English
Published:	University Malaysia Sarawak, (UNIMAS) 2013
Subjects:	Q Science (General) SB Plant culture SD Forestry
Online Access:	http://ir.unimas.my/4455/ http://ir.unimas.my/4455/1/Nucleotide%20polymorphism%20of%20UDP-Glucose..%20%2824%20pages%29.pdf http://ir.unimas.my/4455/2/Nucleotide%20polymorphism%20of%20UDP-Glucose..%20%28fulltext%29.pdf

id	unimas-4455
recordtype	eprints
spelling	unimas-44552017-10-30T06:38:01Z http://ir.unimas.my/4455/ Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba) Ling, Siaw Ching. Q Science (General) SB Plant culture SD Forestry Neolamarckia cadamba (Roxb.) Bosser, also known as Kelampayan, is a medium to large sized, fast growing tree that is belongs to the family of Rubiaceae. Kelampayan is used for industrial plantation and reforestation. It gives the best raw materials for plywood industry as well as pulp and paper industry. UDP-glucose dehydrogenase is an enzyme that catalyzes the conversion of UDP-glucose to UDP-glucuronate, which acts as a key precursor for the synthesis of hemicellulose and pectin in a multistep non-reversible reaction. Hemicellulose and pectin are the important components for cell wall formation. The main objective of this study was to identify nucleotide polymorphisms caused by single nucleotide polymorphisms (SNP) in UDPglucose dehydrogenase gene in Kelampayan. In order to do this, DNA extracted from five Kelampayan trees were first subjected to polymerase chain reaction to obtain the desired UDPGDH sequence. BLASTn analysis was then performed to search for sequence homology. Five UDPGDH sequences were aligned using CLC Free Workbench 6.0 for SNP detection. A total of 3 SNP and 3 InDels had been detected in partial UDP-glucose dehydrogenase sequence. Apart from that, five restriction enzymes which include BssKI, PspGI, StyD4I and MspJI were also detected for three SNP sites. University Malaysia Sarawak, (UNIMAS) 2013 Project Report NonPeerReviewed text en http://ir.unimas.my/4455/1/Nucleotide%20polymorphism%20of%20UDP-Glucose..%20%2824%20pages%29.pdf text en http://ir.unimas.my/4455/2/Nucleotide%20polymorphism%20of%20UDP-Glucose..%20%28fulltext%29.pdf Ling, Siaw Ching. (2013) Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba). [Project Report] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General) SB Plant culture SD Forestry
spellingShingle	Q Science (General) SB Plant culture SD Forestry Ling, Siaw Ching. Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
description	Neolamarckia cadamba (Roxb.) Bosser, also known as Kelampayan, is a medium to large sized, fast growing tree that is belongs to the family of Rubiaceae. Kelampayan is used for industrial plantation and reforestation. It gives the best raw materials for plywood industry as well as pulp and paper industry. UDP-glucose dehydrogenase is an enzyme that catalyzes the conversion of UDP-glucose to UDP-glucuronate, which acts as a key precursor for the synthesis of hemicellulose and pectin in a multistep non-reversible reaction. Hemicellulose and pectin are the important components for cell wall formation. The main objective of this study was to identify nucleotide polymorphisms caused by single nucleotide polymorphisms (SNP) in UDPglucose dehydrogenase gene in Kelampayan. In order to do this, DNA extracted from five Kelampayan trees were first subjected to polymerase chain reaction to obtain the desired UDPGDH sequence. BLASTn analysis was then performed to search for sequence homology. Five UDPGDH sequences were aligned using CLC Free Workbench 6.0 for SNP detection. A total of 3 SNP and 3 InDels had been detected in partial UDP-glucose dehydrogenase sequence. Apart from that, five restriction enzymes which include BssKI, PspGI, StyD4I and MspJI were also detected for three SNP sites.
format	Project Report
author	Ling, Siaw Ching.
author_facet	Ling, Siaw Ching.
author_sort	Ling, Siaw Ching.
title	Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
title_short	Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
title_full	Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
title_fullStr	Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
title_full_unstemmed	Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
title_sort	nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)
publisher	University Malaysia Sarawak, (UNIMAS)
publishDate	2013
url	http://ir.unimas.my/4455/ http://ir.unimas.my/4455/1/Nucleotide%20polymorphism%20of%20UDP-Glucose..%20%2824%20pages%29.pdf http://ir.unimas.my/4455/2/Nucleotide%20polymorphism%20of%20UDP-Glucose..%20%28fulltext%29.pdf
first_indexed	2018-09-06T15:04:56Z
last_indexed	2018-09-06T15:04:56Z
_version_	1610870996765507584

Nucleotide polymorphism of udp-glucose dehydrogenase (udpgdh) from kelampayan (neolamarckia cadamba)

Similar Items