Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters

Tetrodotoxin (TTX) is the toxic principle of puffer fish poisoning, a powerful and specific sodium channel blocker. TTX is exogenous in origin and accumulates in puffer fish through the food chain. Attempts were made to assess the toxicity, and verify toxin properties of marine and brackish puffers...

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Main Authors:	Mohd Din, Monaliza, Mohamad, Samsur
Format:	Article
Language:	English
Published:	2010
Subjects:	AC Collections. Series. Collected works
Online Access:	http://ir.unimas.my/2318/ http://ir.unimas.my/2318/1/Occurrence%20of%20Tetrodotoxin%20in%20Marine%20and%20Brackish%20Puffer%20Fish%20from%20East%20Malaysian%20Waters.pdf

id	unimas-2318
recordtype	eprints
spelling	unimas-23182014-05-06T05:08:25Z http://ir.unimas.my/2318/ Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters Mohd Din, Monaliza Mohamad, Samsur AC Collections. Series. Collected works Tetrodotoxin (TTX) is the toxic principle of puffer fish poisoning, a powerful and specific sodium channel blocker. TTX is exogenous in origin and accumulates in puffer fish through the food chain. Attempts were made to assess the toxicity, and verify toxin properties of marine and brackish puffers Lagocephalus lunaris, Tetraodon nigroviridis and Xenopterus naritus. The presence of TTX in puffer fish tissues were examined and verified by using mouse bioassay, Thin Layer Chromatography (TLC) and Gas Chromatography Mass Spectrometry (GC-MS). Toxicity assessment by mouse bioassay revealed that L. lunaris and T. nigroviridis was toxic (2.5 – 3.8 MU/g and 30.0 – 31.2 MU/g respectively). Meanwhile, thetoxicity scores of toxin extracted from tissues of X. naritus were 2.0 – 2.8 MU/g and the toxin were considered too mild to be detected since the mice did not die within 30 minutes after intra-peritoneally injection. Analysis of TLC showed that tissue extracts of L. lunaris, T. nigroviridis and X. naritus were positive for TTX. From the GC-MS analysis, it was unambiguously concluded that L. lunaris, T. nigroviridis and X. naritus exhibited TTX in their skin and muscle. 2010 Article NonPeerReviewed text en http://ir.unimas.my/2318/1/Occurrence%20of%20Tetrodotoxin%20in%20Marine%20and%20Brackish%20Puffer%20Fish%20from%20East%20Malaysian%20Waters.pdf Mohd Din, Monaliza and Mohamad, Samsur (2010) Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters. Monograph Aquatic Science Colloqium 2010.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English
topic	AC Collections. Series. Collected works
spellingShingle	AC Collections. Series. Collected works Mohd Din, Monaliza Mohamad, Samsur Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters
description	Tetrodotoxin (TTX) is the toxic principle of puffer fish poisoning, a powerful and specific sodium channel blocker. TTX is exogenous in origin and accumulates in puffer fish through the food chain. Attempts were made to assess the toxicity, and verify toxin properties of marine and brackish puffers Lagocephalus lunaris, Tetraodon nigroviridis and Xenopterus naritus. The presence of TTX in puffer fish tissues were examined and verified by using mouse bioassay, Thin Layer Chromatography (TLC) and Gas Chromatography Mass Spectrometry (GC-MS). Toxicity assessment by mouse bioassay revealed that L. lunaris and T. nigroviridis was toxic (2.5 – 3.8 MU/g and 30.0 – 31.2 MU/g respectively). Meanwhile, thetoxicity scores of toxin extracted from tissues of X. naritus were 2.0 – 2.8 MU/g and the toxin were considered too mild to be detected since the mice did not die within 30 minutes after intra-peritoneally injection. Analysis of TLC showed that tissue extracts of L. lunaris, T. nigroviridis and X. naritus were positive for TTX. From the GC-MS analysis, it was unambiguously concluded that L. lunaris, T. nigroviridis and X. naritus exhibited TTX in their skin and muscle.
format	Article
author	Mohd Din, Monaliza Mohamad, Samsur
author_facet	Mohd Din, Monaliza Mohamad, Samsur
author_sort	Mohd Din, Monaliza
title	Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters
title_short	Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters
title_full	Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters
title_fullStr	Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters
title_full_unstemmed	Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters
title_sort	occurrence of tetrodotoxin in marine and brackish puffer fish from east malaysian waters
publishDate	2010
url	http://ir.unimas.my/2318/ http://ir.unimas.my/2318/1/Occurrence%20of%20Tetrodotoxin%20in%20Marine%20and%20Brackish%20Puffer%20Fish%20from%20East%20Malaysian%20Waters.pdf
first_indexed	2018-09-06T14:50:04Z
last_indexed	2018-09-06T14:50:04Z
_version_	1610870061122191360

Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters

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