Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)

Papaya (Carica papaya L.) 'Onefoot' and 'Exotica II' are commercial fruit trees ofthe Caricaceae. The present study was carried out to develop an in vitro shoot regeneration protocol of papaya 'Exotica II' and 'Onefoot'. This study started with development of...

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Main Author:	Chitraa Devi, A/P Krishnan.
Format:	Project Report
Language:	English English
Published:	Universiti Malaysia Sarawak (UNIMAS) 2006
Subjects:	QK Botany SB Plant culture
Online Access:	http://ir.unimas.my/18945/ http://ir.unimas.my/18945/1/Developing%20shoot%20regeneration%20protocol%20..%20%2824%20pages%29.pdf http://ir.unimas.my/18945/2/Developing%20shoot%20regeneration%20protocol%20..%20%28fulltext%29.pdf

id	unimas-18945
recordtype	eprints
spelling	unimas-189452017-12-15T00:51:27Z http://ir.unimas.my/18945/ Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.) Chitraa Devi, A/P Krishnan. QK Botany SB Plant culture Papaya (Carica papaya L.) 'Onefoot' and 'Exotica II' are commercial fruit trees ofthe Caricaceae. The present study was carried out to develop an in vitro shoot regeneration protocol of papaya 'Exotica II' and 'Onefoot'. This study started with development of a protocol for establishing aseptic cultures. Culturing surface sterilized explants on five different PPM concentrated media showed that there was no significant difference in the various concentration of PPM. However, observation showed that explants in PPM 3 ml/l looked normal and healthier than others. Shoot regeneration protocol developed by culturing shoot-tip and axillary-bud explants of 'Exotica II' and 'Onefoot' in three different culture media which incorporated with 3 ml/l PPM and 10 ml/l Tetracycline. Medium B consists of MS medium supplemented with 1.86 mgll NAA + 0.45 mg/I BAP + 10.76 mg/l Kinetin. Medium C consists ofB5 Medium supplemented with 1.86 mgJl NAA + 1.08 mg/I Kinetin and Medium D consists of MS medium supplemented with 0.2 mg/I NAA + 0.5 mg/I BAP. The best result for multiple shoots regeneration was observed from 'Onefoot' shoot-tip explant in Media B and D. Explant in Medium C produced abnormal multiple shoots thus this media was not suitable for multiple shoots regeneration. Therefore, Media Band D could be used to produce multiple shoots regeneration from 'Onefoot' shoot-tip explant. Universiti Malaysia Sarawak (UNIMAS) 2006 Project Report NonPeerReviewed text en http://ir.unimas.my/18945/1/Developing%20shoot%20regeneration%20protocol%20..%20%2824%20pages%29.pdf text en http://ir.unimas.my/18945/2/Developing%20shoot%20regeneration%20protocol%20..%20%28fulltext%29.pdf Chitraa Devi, A/P Krishnan. (2006) Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.). [Project Report] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English English
topic	QK Botany SB Plant culture
spellingShingle	QK Botany SB Plant culture Chitraa Devi, A/P Krishnan. Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)
description	Papaya (Carica papaya L.) 'Onefoot' and 'Exotica II' are commercial fruit trees ofthe Caricaceae. The present study was carried out to develop an in vitro shoot regeneration protocol of papaya 'Exotica II' and 'Onefoot'. This study started with development of a protocol for establishing aseptic cultures. Culturing surface sterilized explants on five different PPM concentrated media showed that there was no significant difference in the various concentration of PPM. However, observation showed that explants in PPM 3 ml/l looked normal and healthier than others. Shoot regeneration protocol developed by culturing shoot-tip and axillary-bud explants of 'Exotica II' and 'Onefoot' in three different culture media which incorporated with 3 ml/l PPM and 10 ml/l Tetracycline. Medium B consists of MS medium supplemented with 1.86 mgll NAA + 0.45 mg/I BAP + 10.76 mg/l Kinetin. Medium C consists ofB5 Medium supplemented with 1.86 mgJl NAA + 1.08 mg/I Kinetin and Medium D consists of MS medium supplemented with 0.2 mg/I NAA + 0.5 mg/I BAP. The best result for multiple shoots regeneration was observed from 'Onefoot' shoot-tip explant in Media B and D. Explant in Medium C produced abnormal multiple shoots thus this media was not suitable for multiple shoots regeneration. Therefore, Media Band D could be used to produce multiple shoots regeneration from 'Onefoot' shoot-tip explant.
format	Project Report
author	Chitraa Devi, A/P Krishnan.
author_facet	Chitraa Devi, A/P Krishnan.
author_sort	Chitraa Devi, A/P Krishnan.
title	Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)
title_short	Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)
title_full	Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)
title_fullStr	Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)
title_full_unstemmed	Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)
title_sort	developing shoot regeneration protocol for selected cultivars of papaya (carica papaya l.)
publisher	Universiti Malaysia Sarawak (UNIMAS)
publishDate	2006
url	http://ir.unimas.my/18945/ http://ir.unimas.my/18945/1/Developing%20shoot%20regeneration%20protocol%20..%20%2824%20pages%29.pdf http://ir.unimas.my/18945/2/Developing%20shoot%20regeneration%20protocol%20..%20%28fulltext%29.pdf
first_indexed	2018-09-06T16:46:51Z
last_indexed	2018-09-06T16:46:51Z
_version_	1610877409080377344

Developing shoot regeneration protocol for selected cultivars of papaya (carica papaya L.)

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