Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products

Ultra high temperature (UHT) treated milk products and formula milk are known to be frequently contaminated with Bacillus cereus. Presence of B. cereus in these milk products is of particular concern considering the majority of consumers are infants and children. Possible sources of contamination ar...

Full description

Bibliographic Details
Main Authors:	Lesley Maurice, Bilung, Ernie, S.R., Kasing, Apun, Radu, Son
Format:	Article
Language:	English
Published:	IFRJ Publisher 2017
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/17414/ http://ir.unimas.my/17414/ http://ir.unimas.my/17414/1/Detection-of-Bacillus-cereus-in-formula-milk-and-ultra-high-temperature-%28UHT%29-treated-milk-products_2017_International-Food-Research-Journal.html

id	unimas-17414
recordtype	eprints
spelling	unimas-174142017-09-05T02:54:41Z http://ir.unimas.my/17414/ Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products Lesley Maurice, Bilung Ernie, S.R. Kasing, Apun Radu, Son Q Science (General) Ultra high temperature (UHT) treated milk products and formula milk are known to be frequently contaminated with Bacillus cereus. Presence of B. cereus in these milk products is of particular concern considering the majority of consumers are infants and children. Possible sources of contamination are contaminated raw milk, cross-contamination during processing, under-processing and mishandling of milk products. This study was conducted to detect the presence of B. cereus in both formula milk (n=12) and UHT milk (n=20) sold in selected retail markets. The approach consisted of enumerating by MPN/g followed by PCR assay aimed at detecting gyrB gene in B. cereus, that encode for the subunit B protein of DNA gyrase (topoisomerase type II). Contamination level of B. cereus in both types of samples examined ranged from < 3 to > 1100 MPN/g. The contamination level of B. cereus was found to be highest in full cream UHT milk (> 1100 MPN/g) and formula milk (> 1100 MPN/g). The PCR analysis showed that 41.7% (5/12) formula milk and 30% (6/20) UHT milk samples were detected with B. cereus, respectively. This is the first report of such study demonstrating the presence of B. cereus in formula milk from Malaysia. Therefore, constant surveillance of these milk products would reduce the potential risk of B. cereus-linked outbreaks. © All Rights Reserved. IFRJ Publisher 2017 Article PeerReviewed text en http://ir.unimas.my/17414/1/Detection-of-Bacillus-cereus-in-formula-milk-and-ultra-high-temperature-%28UHT%29-treated-milk-products_2017_International-Food-Research-Journal.html Lesley Maurice, Bilung and Ernie, S.R. and Kasing, Apun and Radu, Son (2017) Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products. International Food Research Journal, 24 (3). pp. 985-989. ISSN 2231-7546 https://www.researchgate.net/publication/318776668
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Lesley Maurice, Bilung Ernie, S.R. Kasing, Apun Radu, Son Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products
description	Ultra high temperature (UHT) treated milk products and formula milk are known to be frequently contaminated with Bacillus cereus. Presence of B. cereus in these milk products is of particular concern considering the majority of consumers are infants and children. Possible sources of contamination are contaminated raw milk, cross-contamination during processing, under-processing and mishandling of milk products. This study was conducted to detect the presence of B. cereus in both formula milk (n=12) and UHT milk (n=20) sold in selected retail markets. The approach consisted of enumerating by MPN/g followed by PCR assay aimed at detecting gyrB gene in B. cereus, that encode for the subunit B protein of DNA gyrase (topoisomerase type II). Contamination level of B. cereus in both types of samples examined ranged from < 3 to > 1100 MPN/g. The contamination level of B. cereus was found to be highest in full cream UHT milk (> 1100 MPN/g) and formula milk (> 1100 MPN/g). The PCR analysis showed that 41.7% (5/12) formula milk and 30% (6/20) UHT milk samples were detected with B. cereus, respectively. This is the first report of such study demonstrating the presence of B. cereus in formula milk from Malaysia. Therefore, constant surveillance of these milk products would reduce the potential risk of B. cereus-linked outbreaks. © All Rights Reserved.
format	Article
author	Lesley Maurice, Bilung Ernie, S.R. Kasing, Apun Radu, Son
author_facet	Lesley Maurice, Bilung Ernie, S.R. Kasing, Apun Radu, Son
author_sort	Lesley Maurice, Bilung
title	Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products
title_short	Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products
title_full	Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products
title_fullStr	Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products
title_full_unstemmed	Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products
title_sort	detection of bacillus cereus in formula milk and ultra high temperature (uht) treated milk products
publisher	IFRJ Publisher
publishDate	2017
url	http://ir.unimas.my/17414/ http://ir.unimas.my/17414/ http://ir.unimas.my/17414/1/Detection-of-Bacillus-cereus-in-formula-milk-and-ultra-high-temperature-%28UHT%29-treated-milk-products_2017_International-Food-Research-Journal.html
first_indexed	2018-09-06T16:36:18Z
last_indexed	2018-09-06T16:36:18Z
_version_	1610876745052848128

Detection of Bacillus cereus in formula milk and ultra high temperature (UHT) treated milk products

Similar Items