Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci

Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci....

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Main Authors:	Conway, D.J., Machado, Ricardo L.D., Balbir, Singh, Dessert, Patricia, Mikes, Zsuzsanna S., Povoa, Marinete M., Oduola, Ayoade M.J., Roper, Cally
Format:	Article
Language:	English
Published:	Elsevier Science B.V. 2001
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/15849/ http://ir.unimas.my/15849/ http://ir.unimas.my/15849/1/Extreme%20geographical%20fixation%20of%20variation%20in%20the%20Plasmodium%20falciparum%20gamete%20%28abstract%29.pdf

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spelling	unimas-158492017-04-06T01:06:33Z http://ir.unimas.my/15849/ Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci Conway, D.J. Machado, Ricardo L.D. Balbir, Singh Dessert, Patricia Mikes, Zsuzsanna S. Povoa, Marinete M. Oduola, Ayoade M.J. Roper, Cally Q Science (General) Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48 /45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48 /45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48 /45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility. Elsevier Science B.V. 2001 Article PeerReviewed text en http://ir.unimas.my/15849/1/Extreme%20geographical%20fixation%20of%20variation%20in%20the%20Plasmodium%20falciparum%20gamete%20%28abstract%29.pdf Conway, D.J. and Machado, Ricardo L.D. and Balbir, Singh and Dessert, Patricia and Mikes, Zsuzsanna S. and Povoa, Marinete M. and Oduola, Ayoade M.J. and Roper, Cally (2001) Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci. Molecular & Biochemical Parasitology, 115. pp. 145-156. ISSN 0166-6851 http://www.sciencedirect.com/science/article/pii/S016668510100278X
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Conway, D.J. Machado, Ricardo L.D. Balbir, Singh Dessert, Patricia Mikes, Zsuzsanna S. Povoa, Marinete M. Oduola, Ayoade M.J. Roper, Cally Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci
description	Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48 /45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48 /45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48 /45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility.
format	Article
author	Conway, D.J. Machado, Ricardo L.D. Balbir, Singh Dessert, Patricia Mikes, Zsuzsanna S. Povoa, Marinete M. Oduola, Ayoade M.J. Roper, Cally
author_facet	Conway, D.J. Machado, Ricardo L.D. Balbir, Singh Dessert, Patricia Mikes, Zsuzsanna S. Povoa, Marinete M. Oduola, Ayoade M.J. Roper, Cally
author_sort	Conway, D.J.
title	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci
title_short	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci
title_full	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci
title_fullStr	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci
title_full_unstemmed	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci
title_sort	extreme geographical fixation of variation in the plasmodium falciparum gamete surface protein gene pfs48 /45 compared with microsatellite loci
publisher	Elsevier Science B.V.
publishDate	2001
url	http://ir.unimas.my/15849/ http://ir.unimas.my/15849/ http://ir.unimas.my/15849/1/Extreme%20geographical%20fixation%20of%20variation%20in%20the%20Plasmodium%20falciparum%20gamete%20%28abstract%29.pdf
first_indexed	2018-09-06T16:25:10Z
last_indexed	2018-09-06T16:25:10Z
_version_	1610876044124880896

Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48 /45 compared with microsatellite loci

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